细胞过度凋亡是脂多糖抑制细胞滋养细胞侵入能力的重要机制

目的:探讨脂多糖(LPS)对细胞滋养细胞侵入能力的影响及细胞凋亡在此过程中的作用。方法:对体外培养的早孕细胞滋养细胞,给予不同浓度的LPS(0ng/ml、25ng/ml、50ng/ml、100ng/ml、200ng/ml),采用Transwell检测侵入能力的改变,荧光显微镜、电镜观察凋亡细胞的形态学改变,流式细胞仪定量检测细胞凋亡指数,western blot检测Caspase-3、MMP-2和MMP-9的表达。结果:LPS诱导细胞滋养细胞出现过度凋亡,抑制其侵入能力,促进Caspase-3的表达,抑制MMP-2、MMP-9的表达。以上各指标组间差异显著,P值均＜0．01。细胞凋亡指数与其侵入能力以及MMP-2和MMP一9的表达呈负相关,P值均＜0．01。结论:LPS对细胞滋养细胞的侵入能力有显著的抑制作用,细胞过度凋亡是这一作用发生的重要机制。

Excessive Apoptosis is the Key Mechanism of Inhibiting Trophoblasts Invasion by LPS

Objective:To investigate the effects of LPS on the invasion of trophoblasts and the role of apoptosis in the above procession.Methods:Early pregnant cytotrophoblasts were cultured and treated with different doses of LPS(0ng/ml,25ng/ml,50ng/ml,100ng/ml and 200ng/ml)in vitro.Transwell was applied to test the ability of cytotrophoblast invasion.Aapoptosis of trophoblasts was determined by fluorescence microscope,transmission electron microscope,and flow cytometry.The expression of Caspase-3,MMP-2,and MMP-9 was determined by Western blot.Results:LPS induced excessive apoptosis of trophoblasts,promoted the expression of Caspase-3,and inhibited the expression of MMP-2 and MMP-9.There were significant differences between each two groups (P＜0.01).There was a negative relation between apoptosis index and its invasive ability and the expression of MMP-2 and MMP-9(P＜0.01).Conclusion:LPS deeply inhibited trophoblast invasion and apoptosis was the key mechanism.