| Hfq对伤寒沙门菌高渗应激早期基因表达的调节 |
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| 引用本文： | 谢新民,李安平,杜鸿,茅凌翔,罗哲,王敏,黄新祥. Hfq对伤寒沙门菌高渗应激早期基因表达的调节[J]. 江苏大学学报(医学版), 2010, 20(1): 1-5 |
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| 作者姓名： | 谢新民 李安平 杜鸿 茅凌翔 罗哲 王敏 黄新祥 |
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| 作者单位： | 1. 江苏大学生命科学研究院,江苏,镇江,212013
2. 江苏大学基础医学与医学技术学院生物化学研究室,江苏,镇江,212013 |
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| 摘 要： | 目的：探查伤寒沙门菌hfq基因在高渗应激早期对其他基因表达的影响。方法：用自杀质粒介导的同源重组方法制备伤寒沙门菌hfq基因缺陷变异株；利用伤寒沙门菌全基因组芯片技术，比较伤寒沙门菌野生株和咆向基因缺陷变异株在高渗应激早期的基因表达谱差异，并选择部分表达差异基因进行实时定量PCR验证。结果：伤寒沙门菌hfq基因缺陷变异株制备成功；基因表达谱比较分析结果表明伤寒沙门菌hfq基因缺陷变异株在高渗应激早期有62个基因表达上调，有32个基因表达下调。实时定量PCR结果与芯片分析一致。结论：Hfq作为一个调节因子在伤寒沙门菌高渗应激早期对基因表达起重要调节作用。
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| 关 键 词： | 伤寒沙门菌 hfq 高渗应激 基因芯片分析 |
Influence of Hfq on gene expression regulation of Salmonella enterica serovar Typhi at earlier stage of hyperosmotic stress |
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| XIE Xin-min,LI An-ping,DU Hong,MAO Ling-xiang,LUO Zhe,WANG Min,HUANG Xin-xiang. Influence of Hfq on gene expression regulation of Salmonella enterica serovar Typhi at earlier stage of hyperosmotic stress[J]. Journal of Jiangsu University Medicine Edition, 2010, 20(1): 1-5 |
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| Authors: | XIE Xin-min LI An-ping DU Hong MAO Ling-xiang LUO Zhe WANG Min HUANG Xin-xiang |
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| Affiliation: | XIE Xin-min1,LI An-ping2,DU Hong2,MAO Ling-xiang2,LUO Zhe1,WANG Min2,HUANG Xin-xiang2(1.School of Life Science,2.Department of Biochemistry,School of Medical Science , Laboratory Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China) |
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| Abstract: | Objective:To explore the influence of Hfq on gene expression regulation of S.enterica serovar Typhi at early-stage of hyperosmotic stress.Methods:The hfq deleted mutant of S.enterica serovar Typhi was prepared by homologous recombination mediated by suicide plasmid;the difference of gene expression profiles between the wild strain and the hfq mutant at early-stage of hyperosmotic stress was investigated by genomic assay;qRT-PCR was performed to validate the results of microarray assay. Results:The hfq delet... |
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| Keywords: | hfq |
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