大豆黄酮对人肝癌SMMC7721细胞己糖激酶活性和癌干细胞CD133蛋白水平的影响 |
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引用本文: | 徐衍,沈永杰,安玉会,杨丽君,马乾坤,李莉,胡博文,石晓娟,孙静蕾. 大豆黄酮对人肝癌SMMC7721细胞己糖激酶活性和癌干细胞CD133蛋白水平的影响[J]. 河南职工医学院学报, 2013, 25(3): 240-244 |
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作者姓名: | 徐衍 沈永杰 安玉会 杨丽君 马乾坤 李莉 胡博文 石晓娟 孙静蕾 |
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作者单位: | 徐衍 (郑州大学基础医学院生物化学与分子生物学教研室,郑州,450052); 沈永杰 (郑州大学基础医学院生物化学与分子生物学教研室,郑州,450052); 安玉会 (郑州大学基础医学院生物化学与分子生物学教研室,郑州,450052); 杨丽君 (郑州大学基础医学院生物化学与分子生物学教研室,郑州,450052); 马乾坤 (郑州大学基础医学院生物化学与分子生物学教研室,郑州,450052); 李莉 (郑州大学基础医学院生物化学与分子生物学教研室,郑州,450052); 胡博文 (郑州大学基础医学院生物化学与分子生物学教研室,郑州,450052); 石晓娟 (郑州大学基础医学院生物化学与分子生物学教研室,郑州,450052); 孙静蕾 (郑州大学基础医学院生物化学与分子生物学教研室,郑州,450052); |
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摘 要: | 目的探讨大豆黄酮对肝癌SMMC7721细胞糖代谢和癌干细胞CD133基因表达的影响。方法培养人肝癌SMMC7721细胞并用大豆黄酮处理。MTT法测定细胞的成活率,用试剂盒法测定人肝癌SMMC7721细胞中的己糖激酶活性,并用WesternBlot方法测定癌干细胞CD133蛋白的水平。结果大豆黄酮对人肝癌SMMC7721细胞增殖具有抑制作用,同时,可降低人肝癌SMMC7721细胞中己糖激酶活性和癌干细胞CD133蛋白的水平。结论大豆黄酮能抑制人肝癌SMMC7721细胞的增殖,降低人肝癌SMMC7721细胞中己糖激酶活性和癌干细胞CD133蛋白的水平。
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关 键 词: | 大豆黄酮 人肝癌干细胞 MTT CD133蛋白 Western blot |
Influence of Soybean Flavone to the Activity of Hexose Kinase in SMMC7721 Cell Lines of Human Liver Cancer and the Level of CD133 Protein in Cancer Stem Cell |
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Affiliation: | XU Yan, SHEN Yong-jie, AN Yu-hui, et al. (Department of Biochemistry and Molecular Biology of Basic Medical College of Zhengzhou University, Zhengzhou Henan 450052, China) |
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Abstract: | Objective To explore the influence of soybean flavone to the activity of hexokinase in SMMC7721 cell lines of human liver cancer and the level of CD133 protein in cancer stem cell. Methods Culturing SMMC7721 cells of human liver cancer, and treating them with soybean flavone. The num- bers of living cells were detected by MTT method. The activity of hexokinase in SMMC7721 cell lines of human liver cancer and the level of CD133 protein in cancer stem cell were respectively measured by Kit of hexokinase and by Western blot method. Results Soybean flavone had an inhibition to SMMC7721 cell lines of human liver cancer. At the same time, soybean flavone also lowered the activity of hexoki- nase in SMMC7721 cell lines of human liver cancer and the level of CD133 protein in cancer stem cell. Conclusion Soybean flavone can inhibit the proliferation of SMMC7721 cell lines of human liver cancer and the activity of hexokinase in the cells,and lower the level of CD133 protein in cancer stem cells. |
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Keywords: | soybean flavone human liver cancer stem cell MTT hexokinase CD133 protein West- ern blot |
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