山楂醇提物腹腔注射对辐射损伤小鼠保护作用的研究

目的 研究山楂醇提物腹腔注射对辐射损伤小鼠的保护作用。方法 昆明种雄性小鼠60只被分为五组:正常对照、放射对照、药物高、中、低剂量组。各药物组腹腔内注射一次药物,其余两组给予生理盐水,1h后实验组小鼠用⁶⁰Coγ射线6Gy照射。分别在照射前4h、照后6d、照后12d称量小鼠体重、检测血象三次。在照后12d,处死小鼠,测股骨骨髓DNA含量、骨髓有核细胞数。结果 ①高剂量组8只小鼠注射药物后腹腔感染出血死亡,其余实验小鼠均存活。②同放射对照组比,三种浓度的山楂醇提物可以促进受辐射损伤小鼠外周血白细胞、血小板数的恢复。结论 山楂醇提物腹腔注射可促进受辐射损伤小鼠造血功能的恢复,对骨髓DNA及有核细胞有一定的保护作用。

Study of Radioprotective Effect of Crataegus on Mouse by Intraperitoneally Injection

Objective To explore the radioprotective effect of Crataegus on Mouse by intraperitoneally injection.Methods 60 mice were randomly divided into five groups:normal control,irradiation,irradiation+high dosage,irradiation+middle dosage,irradiation +low dosage.The latter three groups were injected intraperitoneally with Crataegus extract 1 hour before they were irradiated by ⁶⁰Coγ.The others were given physiological brine.The body weight and Peripheral blood routine were measured at 4h before irradiation and 6、12 days after irradiation.DNA content and quantity of nucleocyte in bone marrow were observed at 12 days after irradiation.Results ①After injection 8 mice died of infecton and hemorrhage in high dose group,and all the others survived.②In contrast with the irradiation group,the extract of Crataegus in three different concentrations has the effect of promoting the recovery of WBC,PLT counts in peripheral blood of mice with radiation injury mice.Conclusion The extract of Crataegus can promote recovery of hematological function and protect the bone marrow DNA and nucleocyte of radiation injury mice.