SARS-CoV抗原化学发光免疫分析法的应用

目的建立一种可用于严重急性呼吸综合征(SARS)早期诊断的简便、快速、灵敏的实验室检测方法。方法采用化学发光免疫分析方法(chemiluminescenceimmunosaasy,CLIA)测定12种不同病毒培养上清和人血中SARS冠状病毒核衣壳蛋白(nucleocapsidprotein)抗原(SARS-CoV-N)。结果7种不同SARS毒株测定均为阳性,12种非SARS毒株测定均为阴性,最低可检出核酸定量为6.3×101PFU/mL的SARS-CoV培养上清;测定63例临床确诊SARS的阳性血清样本,发病6~10d的样本检出率最高,可达100%;测定1066例正常人样本,99.35%为阴性。结论SARS-CoV的CLIA的灵敏度和特异性较高,并且简便、快速,在SARS的早期诊断中展示了良好的应用前景。

Chemiluminescence Immunoassay for Detection of SARS-CoV Protein Antigen

Objective To establish a laboratory method for rapid, simple and sensitive diagnosis of SARS. Methods Culture supernote of 34 viruses strains and sera of SARS patients were assayed by CLIA. Results The experiments indicated high specificity and sensitivity to viruses strains, reactivity with 6 SARS strains and not cross-reactivity with 11 others strains, the detectability was on more then 6.3×10¹ PFU/mL of SARS-CoV strain. For the 63 SARS patients, positive rate was found to be 100% during the disease day of 6～10 and for the 1 066 normal sample, negative rate was 99.35%. Conclusion The sensitivity and specificity of CLIA for SARS-CoV detection is higher satisfying. It is rapid and simple. It might be used to earliest diagnosis of SARS-CoV infection.