引种栽培与原产地珐菲亚中总皂苷的微波提取及含量测定

目的建立比色法测定珐菲亚中总皂苷的含量,为珐菲亚的质量控制提供依据。方法以齐墩果酸为对照品,用香草醛-冰醋酸,高氯酸试剂显色反应,在542 nm处有最大吸收。分别考察回流法提取法、超声波萃取法及微波萃取法。选择最优提取工艺,对样品进行含量测定。结果齐墩果酸在0.005 8~0.029 mg/ml范围内呈良好的线性关系。平均回收率和RSD分别为100.05%和1.31%。方法学考察各项指标均符合要求。结论该法简便、准确,稳定性、重现性好。

The Microwave Extraction and Quantitative Analysis of Total Saponins in Both Introduced and Original Pfaffia paniculata

Objective To establish the colorimetric assay for the content determination of total saponins in Pfaffia paniculata,which can be used for the quality control of Pfaffia paniculata.Methods With oleanolic acid as control substance,and vanillin-glacial acetic acid and perchloric acid were used as analytical reagents,the peak absorption was 542nm.The backset current extraction,ultrasonic-assisted extraction and microwave extraction were tested to select the optimum extraction and the quantitative analysis of specimen was made.Results In the range of 0.005 8~0.029 mg/ml oleanolic acid were in direct proportion,the mean recovery rate was100.05% and relative standard deviation RSD was 1.31%.The indexes were up to the standard of methodology.Conclusion The method is simple and convenient.It has high accuracy and good reappearance.