Preparation of anti-idiotypic antibody against avian influenza virus subtype H9 |
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Authors: | Li Baoquan Peng Jun Niu Zhongxiang Yin Xunhe Liu Faxiao |
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Institution: | College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China. |
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Abstract: | To generate monoclonal anti-idiotypic antibodies (mAb2) against avian influenza virus subtype H9 (H9 AIV), BALB/c mice were immunized with purified chicken anti-H9-AIV IgG and the splenocytes of immunized mice were fused with myeloma cells NS-1. Hybridoma cells were screened by indirect enzyme-linked immunosorbent assays with both chicken and rabbit anti-H9-AIV IgG as coating antigens. One hybridoma cell clone secreting monoclonal antibody against idiotypes shared by both chicken and rabbit anti-H9-AIV IgG was established. Experiments demonstrated the mAb2 was able to inhibit the binding of hemagglutinin to anti-H9-AIV IgG and to induce chickens to generate hemagglutination inhibition antibodies, indicating this anti-species-sharing-idiotypic antibody bore the internal image of hemagglutinin on avian influenza virus. |
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Keywords: | avian influenza virus anti-idiotypic antibody idiotype |
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