HPLC测定北五味子果实、藤茎超临界萃取物中木脂素的含量

 目的 采用HPLC同时测定北五味子果实、藤茎超临界萃取物中五味子甲素、五味子乙素、五味子醇甲的含量。方法 采用ODS-C₁₈(4.6 mm×250 mm,5 μm),以甲醇-水（75∶25）为流动相,流速：1.0 mL·min-1,检测波长为254 nm。结果 五味子甲素、五味子乙素、五味子醇甲的线性范围分别为0.10～2.00 μg(r=0.999 4)、0.10～2.00 μg(r=0.999 7)、0.12～2.40 μg (r=0.999 2),平均加样回收率分别为99.11%、101.18%、101.23%（RSD分别为1.57%、2.68%、2.80%)（n＝10）。结论 本方法简便准确、重现性好,能有效测定北五味子果实、藤茎超临界萃取物中五味子甲素、五味子乙素、五味子醇甲的含量。

Determination of Lignans in Supercritical Carbon Dioxide Extract from Fructus and Caulis Schisandrae chinensis by HPLC

OBJECTIVE To establish a HPLC method for the determination of deoxyschizandrin, γ-schizandrin and schizandrin in supercritical carbon dioxide extract from Fructus and Caulis Schisandra chinensis. METHODS The analysis was performed on ODS-C₁₈ column (4.6 mm×250 mm,5 μm) with the mobile phase consisting of methanol-water (75∶25) as mobile phase,flow rate of 1.0 mL·min-1. The detection wavelength was at 254 nm.RESULTS Satisfactory linearities of deoxyschizandrin,γ-schizandrin and schizandrin were shown in the range of 0.10-2.00 μg (r=0.999 4), 0.1-2.00 μg (r=0.999 7)、0.12-2.40 μg (r=0.999 2). The average recovery were 99.11%, 101.18% and 101.23%（RSD were 1.57%, 2.68% and 2.80%）(n=10). CONCLUSION The method is simple,accurate,highly sensitive and reproducible.It can be effectly used for the determination of deoxyschizandrin,γ-schizandrin and schizandrin in supercritical carbon dioxide extract from Fructus and Caulis Schisandra chinensis.