反相高效液相色潽法测定不同月份高良姜中高良姜素的含量

 目的 建立反相高效液相色谱法测定不同月份高良姜中高良姜素含量的方法。方法 采用液相色谱-质谱联用(LC-MS) 进行峰识别和分离度判断，获得优化的色谱分离条件：Shim-pack VP-ODS柱（2.0 mm×250 mm，5 μm），流动相A为0.1％HAc水溶液，流动相B为15％乙腈+40％甲醇+45％四氢呋喃，梯度洗脱程序为0～20 min、39%～61%B；20～30 min、61%～100%B；流速0.2 mL·min-1，柱温35 ℃，检测波长280 nm。结果 高良姜素色谱峰的分离度大于1.5，高良姜素在0.020～0.64 g·L-1内呈良好线性，r=0.999 8；平均回收率为100.7%，RSD为2.82%（n=6）。结论 本方法准确、精密度高、专属性好，可用于高良姜道地药材及其制剂的质量控制。

Determination of the Content of Galangin in Alpinia officinarum Hance Harvested in Different Monthes by RP-HPLC

OBJECTIVE To establish a RP-HPLC method for the determination of the content of galangin in Alpinia officinarum Hance. METHODS An optimum HPLC conditions with binary mobile phase system were obtained. The samples were separated on a Shim-pack VP-ODS column（2.0 mm×250 mm，5 μm）. The mobile phase consisted of 0.1％HAc aqueous solution as phase A and 15％ acetonitrile+40％ methanol+45％ tetrafuran as phase B. Gradient elution program was as followed: 0-20 min， 39%-61%B; 20-30 min， 61%～100%B; 30-40 min， 100%B. The flow rate was 0.2 mL·min-1 ， column temperature was 35 ℃ and UV was at 280 nm. RESULTS The chromatographic resolution of glangin was above 1.5. A good linearity was obtained with the correlation coefficients ranged of 0.020-0.64 g·L-1 (r=0.999 8).The mean recovery was 100.7%，RSD was 2.82%（n=6）. CONCLUSION The method is reliable，accurate and specific， which can be used for the quality control of Alpinia officinarum Hance and its products.