| JAK2/STAT3信号转导通路参与调节IL-6介导的肺腺癌细胞株中MMP-10的表达 |
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| 引用本文： | 银培基,狄冬梅,罗光华,郑璐,魏江,张俊,施媛萍,张继琛,张晓膺.JAK2/STAT3信号转导通路参与调节IL-6介导的肺腺癌细胞株中MMP-10的表达[J].中华临床医师杂志(电子版),2009,3(6):18-22. |
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| 作者姓名： | 银培基 狄冬梅 罗光华 郑璐 魏江 张俊 施媛萍 张继琛 张晓膺 |
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| 作者单位： | 1. 苏州大学附属第三医院心胸外科,江苏常州,213003
2. 苏州大学附属第三医院综合实验室,江苏常州,213003 |
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| 基金项目： | 常州市科技局指导性项目 |
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| 摘 要： | 目的研究Janus激酶2（JAK2）及信号转导和转录激活子3（STAT3）途径对IL-6介导的肺腺癌细胞（A549）中基质金属蛋白酶10（MMP-10）表达调节的作用,探讨MMP-10的调节机制。方法待生长状况良好的A549细胞融合生长至50%～70%时,分别用0,12.5,25,50ng/mlIL-6及AG490（JAK2特异性抑制剂）＋25ng/mlIL-6处理A549细胞24h后,采用实时RT-PCR技术检测JAK2、STAT3和MMP-10mRNA的表达水平;应用Westernblot检测MMP-10蛋白的表达水平。结果25ng/mlIL-6组STAT3mRNA表达水平较0ng/ml组高43.87%（P〈0.05）,AG490＋25ng/mlIL-6组较25ng/mlIL-6组低36.73%（P〈0.01）;AG490＋25ng/mlIL-6组MMP-10mRNA表达水平较25ng/mlIL-6组高43.21%（P〈0.01）;12.5,25,50ng/mlIL-6组的MMP-10蛋白水平显著高于0ng/ml组（P〈0.05）,且峰值出现在25ng/ml组;与25ng/mlIL-6组相比,MMP-10蛋白水平在AG490＋25ng/mlIL-6组明显降低（P〈0.05）。结论JAK2/STAT3信号转导途径可能参与调节IL-6介导的肺癌细胞株A549中MMP-10的表达。
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| 关 键 词： | 肺肿瘤 Janus激酶2 STAT3转录因子 基质金属蛋白酶10 白细胞介素6 |
Up-regulation of MMP-10 expression is mediated by JAK2/STAT3 signaling pathway in human lung adenocarcinoma cell line |
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| YIN Pei-ji,DI Dong-mei,LUO Guang-hua,ZHENG Lu,WEI Jiang,ZHANG Jun,SHI Yuan-ping,ZHANG Ji-chen,ZHANG Xiao-ying.Up-regulation of MMP-10 expression is mediated by JAK2/STAT3 signaling pathway in human lung adenocarcinoma cell line[J].Chinese Journal of Clinicians(Electronic Version),2009,3(6):18-22. |
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| Authors: | YIN Pei-ji DI Dong-mei LUO Guang-hua ZHENG Lu WEI Jiang ZHANG Jun SHI Yuan-ping ZHANG Ji-chen ZHANG Xiao-ying |
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| Institution: | . (Department of Thoracic and Cardiovascular Surgery, The Third Affiliated Hospital of Suzhou University, Changzhou 213003, China) |
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| Abstract: | Objective To investigate the role of JAK2/STAT3 in the expression of MMP-10 in human lung adenocarcioma cell line and explore the molecular regulatory mechanism of MMP-10 expression. Methods A549 cells were seeded in six-well cell culture clusters, and were grown to 50% - 70% confluence. 0,12. 5,25,50 ng/ml IL-6 and AG490 ＋ 25 ng/ml IL-6 were given to A549 cells, respectively. The expression of JAK2, STAT3 and MMP-10 mRNA were detected with real-time RT-PCR assay. MMP-10 protein was determined by Western blot. Results In A549 cells,STAT3 mRNA levels at 25 ng/rnl IL-6 was higher than at 0 ng/ml IL-6 （ P 〈 0. 05 ）. 50 ixmol/L AG490, a specific JAK2 inhibitor, together with 25 ng/ml IL-6 decreased STAT3 mRNA expression by 36. 73%, compared with 25 ng/ml IL-6 （ P 〈 0. 01 ）. On the contrary, MMP-10 mRNA levels in 3.549 cells treated with 50/.Lmol/L AG490 ＋ 25 ng/ml IL-6 were significantly higher than with 25 ng/ml IL-6 （P 〈 0. 01 ）. MMP-10 protein levels at 12. 5,25,50 ng/ml IL-6 were significantly increased compared with 0 ng/ml IL-6 （P 〈 0. 05 ）. 50 μmol/L AG490, together with 25 ng/ml IL-6 reduced MMP-10 protein expression compared with 25 ng/ml IL-6 （P 〈 0. 05）. Conclusions It is suggested that the JAK2/ STAT3 pathway might be involved in the expression of MMP-10 in A549 cells. |
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