cyclin D1和CDK4在口腔正常上皮、炎症及鳞癌中表达的定量分析

目的 通过检测口腔粘膜的正常上皮、非特异性炎症上皮及鳞癌中cyclin D1与CDK4的表达，探讨其在上皮组织不同状态中的变化及意义。方法 用临床病理标本，设立对照，选择cyclin D1和CDK4抗体免疫组化染色。结果 cyclin D1和CDK4在正常口腔上皮与口腔鳞癌上皮、非特异性炎症与口腔鳞癌上皮中的表达间有统计学差别（P＜0．05），正常组与炎症组间无差别，cyclin D1及CDK4在口腔鳞癌上皮中过表达（P＜0．05）。结论 cyclin D1与CDK4过表达的机制可能由于基因扩增或其他因素使其蓄积，口腔鳞癌组分级间的差异性提示其可作为肿瘤分级的评价指标之一。

Quantitative analysis of expression of cyclin D1 and CDK4 in normal, inflammatory and malignant epithelia of cheek mucosa

AIM To evaluate the expression and significance of cyclinD1/CDK4 in normal epithelia (N), inflammatory (IF) and squamous cell cacinoma (SCC) of cheek mucosa. METHODS Oringinal pathology specimens were collected cut and immunostained by cyclin D1 monoclonal antibody andCDK4 polyclonal antibody, using the avidin biotin peroxidase complex technique (ABC). RESULTS There was statistical significance between the N group and SCC group, but not between the N group and IF group. Cyclin D1 and CDK4 were overexpressed in SCC. CONCLUSION Overexpression of cyclinD1 and CDK4 in SCC may be due to the gene amplification and/or other related factors. The variations of cyclin D1and CDK4 in different subgroups of SCC may be a helpful indicator for tumor grading.