99mTc‐labeled rHuEpo for imaging of the erythropoietin receptor in tumors |
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| Authors: | Xiaoqing Zhuang Dan Zhao Pengfei Yang Yingqin Jia Rui Liang Qian Zhao Chunlei Han Joseph M. Kinsella Ruilong Sheng Juan Li |
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| Affiliation: | 1. General Hospital of Ningxia Medical University, Yinchuan, China;2. Turku PET Centre, Turku University Hospital and University of Turku, Turku, Finland;3. Department of Bioengineering, McGill University, Quebec, Canada;4. CAS Key Laboratory for Organic Functional Materials, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China;5. CQM‐Centrode Quimica da Madeira, Universidade da Madeira, Funchal, Madeira, Portugal |
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| Abstract: | To analyze erythropoietin receptor (EpoR) status in tumors, recombinant human erythropoietin (rHuEpo) was labeled with 99mTc by 99mTc‐centered 1‐pot synthesis, resulting in high radiochemical purity, stability, and biological activity. Both in vitro cell culture experiments and biodistribution studies of normal rats demonstrated successful EpoR targeting. The biodistribution of labeled rHuEpo in a NCI‐H1975 xenograft model showed tumor accumulation (tumor‐to‐muscle ratio, 4.27 ± 1.77), confirming the expression of active EpoR in tumors. Thus, as a novel single positron emission computerized tomography tracer for the imaging of EpoR expression in vivo, 99mTc‐rHuEpo is effective for exploring the role of EpoR in cancer growth, metastasis and angiogenesis. |
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| Keywords: | 99mTc erythropoietin receptor molecular imaging radiotracer rHuEpo |
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