| 睾酮异鲁米诺标记物的化学发光测定 |
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| 引用本文： | 张翊 徐兆昌. 睾酮异鲁米诺标记物的化学发光测定[J]. 蚌埠医学院学报, 1995, 20(3): 182-183 |
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| 作者姓名： | 张翊 徐兆昌 |
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| 作者单位： | 上海医科大学分析化学教研室 |
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| 摘 要： | 本文研究睾酮异鲁米诺标记的化学发光测定，探讨氢氧化钠、过氧化氢、微晶过氧化酶用量对该标记物化学发光的影响，得出各试剂的最佳浓度，氢氧化钠为６ｍｏｌ／Ｌ，过氧化氢为０．２％，微晶过氧化酶为３０μｇ／ｍｌ。发光检测限为０．２１ｐｇ／管。
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| 关 键 词： | 睾酮 异鲁米诺 发光标记 化学发光测定 |
TUDY OF A CHEMILUMINESCENT ASSAY FOR THE LIGHTEMIT TING TRACE OF TESTOSTERONE |
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| Zhang Yi,Xu Zhaochang,Lu Minglian. TUDY OF A CHEMILUMINESCENT ASSAY FOR THE LIGHTEMIT TING TRACE OF TESTOSTERONE[J]. Journal of Bengbu Medical College, 1995, 20(3): 182-183 |
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| Authors: | Zhang Yi Xu Zhaochang Lu Minglian |
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| Abstract: | We studied a chemiluminescent assay for the lightemitting trace of testosterone and made imvestigations on the relation of the comcentration of MaOH,H2O2 and microperoxidase to the light strength especially.The suitable comcentration for the asay was:NaOH 6mol/L,H2O20.2%,microperoxidase 30ug/ml.The limit of detection of the trace was 0.21pg/tube(0.3fmol/tube). |
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| Keywords: | testosterone testosterone coupled isoluminol chemiluminescent |
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