| 肝癌细胞侵袭行为与其流变特性相关 |
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| 引用本文： | 吴泽志,罗向东,王宪航,张钢,龙勉,蔡绍皙.肝癌细胞侵袭行为与其流变特性相关[J].生物医学工程学杂志,2000,17(2):133-138. |
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| 作者姓名： | 吴泽志 罗向东 王宪航 张钢 龙勉 蔡绍皙 |
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| 作者单位： | 1. 重庆大学,生物工程学院,国家教育部生物力学及生物流变学开放实验室,重庆,400044
2. 第三军医大学,烧伤研究所,重庆,400038 |
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| 基金项目： | 国家自然科学基金!39500037,39370198,19732003-2 |
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| 摘 要： | 以原代肝细胞、肝细胞癌（Ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ，ＨＣＣ）细胞及对人工基底膜Ｍａｔｒｉｇｅｌ具有侵袭能力的ＨＣＣ细胞（ＨＣＣ－Ｉｎｖ细胞）为研究对象，研究了三种细胞的粘弹特性、与胶原蛋白Ⅰ的粘附力学特性及与肝血窦内皮细胞（Ｌｉｖｅｒ ｓｉｏｎｓｏｉｄａｌ ｅｎｄｏｔｈｅｌｉａｌ ｃｅｌｌ，ＬＥＣ）的粘附力学特性。结果发现，ＨＣＣ细胞的上述流变特性较之于肝细胞发生明显的变化，
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| 关 键 词： | 肝细胞癌 胶原蛋白Ⅰ Matrigel 细胞流变学 |
Invasion Behavior of Hepatocellular Carcinoma Cells Correlates with Their Rheological Properties |
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| Wu Zezhi,Luo Xiangdong,Wang Xianhang,Zhang Gang,Long Mian,Cai Shaoxi.Invasion Behavior of Hepatocellular Carcinoma Cells Correlates with Their Rheological Properties[J].Journal of Biomedical Engineering,2000,17(2):133-138. |
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| Authors: | Wu Zezhi Luo Xiangdong Wang Xianhang Zhang Gang Long Mian Cai Shaoxi |
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| Institution: | College of Bioengineering, Chongqing University, Chongqing 400044. |
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| Abstract: | We investigated the viscoelasticity, mechanics of adhesion to collagen I coated surfaces and mechanics of adhesin to liver sinosoidal endothelial cells(LECs) among primary culture human hepatocytes, hepatocellular carcinoma(HCC) cells as well as HCC-Inv cells, namely HCC cells capable of invading the artificial basement membrane, Matrigel. The results showed that the above-mentioned rheological properties of HCC cells differed obviously from those of normal hepatocytes. HCC-Inv cells exhibited higher viscoelastic coefficients, higher adhesion forces to collagen I coated surfaces as well as higher adhesion forces to LECs than those of normal hepatocytes and HCC cells. Immunocytochemistry showed the differences in cell morphology and cytoskeleton structure between HCC cells and HCC-Inv cells. A brief discussion was presented in relation to the changes in cell rheological properties of hepatocellular carcinoma cells and their pathological significance. |
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| Keywords: | Matrigel |
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