gax基因对血管平滑肌细胞中PCNA和CDK2表达的影响

目的研究增殖细胞核抗原(PCNA)和细胞周期蛋白依赖性激酶2(CDK2)在gax基因抑制血管平滑肌细胞(VSMC)增殖中的作用。方法以携带大鼠gax基因表达序列的重组腺病毒载体(AdCMV-gax)转染VSMC后，检测gax、PCNA和CDK2的表达及^3H-TdR掺入量的变化。结果AdCMV-gax转染后，VSMC中Gax蛋白的表达比转染前显著增高；AdCMV-gax转染后VSMC的PCNA和CDK2表达较未转染组显著降低；AdCMV-gax转染使VSMC的。H-TdR掺人量显著降低。结论gax基因抑制VSMC增殖的机制与其抑制tK2NA和CDK2的表达有关。

Effect of gax gene on the expression of PCNA and CDK2 in vascular smooth muscle cells

Objective To investigate the role of proliferating cell nuclear antigen (PCNA) and cyclin dependent kinase 2 (CDK2) in inhibition of vascular smooth muscle cells (VSMCs) proliferation by gax gene. Methods After being transfected by AdCMV-gax, the expression of gax, PCNA and CDK2 in VSMCs were assessed. The effect of gax overexpressions on VSMCs proliferation was observed by 3 H-thymidine incorporation. Results After AdCMV-gax was transfected to VSMCs, the level of Gax protein expression was significantly higher than that before transfection. PCNA and CDK2 expressions were decreased after VSMCs were transfacted with AdCMV-gax. The level of 3 H-thymidine incorporation was decreased significantly in with AdCMV-gax transfected VSMCs compared with that in non-transfected VSMCs. Conclusion The mechanism of inhibition of VSMCs proliferation by gax gene is related with depression of expression of PCNA and CDK2.