本事琥珀散对子宫内膜异位症大鼠模型子宫内膜VEGF蛋白含量的影响

目的:通过比较本事琥珀散治疗前后子宫内膜异位症(EMs)大鼠模型在位和异位内膜VEGF蛋白含量的影响,探讨本事琥珀散在EMs血管形成中的作用机制。方法:采用自体内膜移植法建立EMs大鼠模型,给药4周后Western Blot方法检测子宫内膜VEGF蛋白含量。结果:与疾病模型组比较,本事琥珀散组异位内膜VEGF蛋白含量明显降低,差异均具有统计学意义(P<0.05)。结论:本事琥珀散能够通过降低EMs异位内膜VEGF蛋白含量,抑制异位内膜新生血管形成,从而对EMs起到一定的治疗作用。

Effects of BenShiHuPoSan on Th1/Th2 Drift in Endometriosis Model Rats

Objective ： By comparing the changes of VEGF in endometriosis model rats＇ ectopic endometrium in before and after using BenShiHuPoSan （ BSHPS）, we discuss about the effects of BSHPS on angiogenesis in Endometriosis. Methods： First, establish the rat model of EMs by transplanting autologous endometrium to the abdominal wall. Then divide the rats randomly into 5 groups： normal group, pseudo operation group, disease group, BSHPS group and danazol group. Finally, detect the contents of VEGF in endometrium by Western Blot, after dosing for 4 weeks. Results ： Compared with the normal group, the content of VEGF in ectopic endometrium of disease group is much higher. Compared with disease group, the content of VEGF in ectopic endometrium of BSHPS group is significantly reduced （P 〈 0.05）. Conclusion： BSHPS can reduces the content of VEGF in EMs model rats＇ ectopic endometrium, and inhibites angiogenesis to treat EMs.