睫状神经营养因子对周围神经损伤后再生的影响

目的 研究睫状神经营养因子（ＣＮＴＦ）对周转神经损伤后再生的影响。方法 用硅胶管桥接大鼠双侧坐骨神经缺损，形成神经再生室。左侧室内注入基因重组人ＣＮＴＦ，右侧注入生理盐水（ＮＳ）。术后１４、３０、６０和９０天取材，行大体、光镜、电镜观察。术后９０天行轴突图像分析、电生理检测及霍乱毒素－辣根过氧化物酶（ＣＢ－ＨＲＰ）逆行追踪。结果 与对照侧相比，实验侧再生有髓神经纤维数目较多、轴突较粗且髓鞘较厚，复合肌肉动作电位（ＣＭＡＰ）的潜伏期较短、神经传导速度较快且波幅较高。实验侧ＣＢ－ＨＲＰ标记的脊髓前角运动细胞数明显多于对照侧。结论 外源性ｈＣＮＴＦ有明显促进周围神经再生作用。

Effect of ciliary neurotrophic factor on the regeneration of transected peripheral nerve

Objective:To investigate the effect of ciliary neurotrophic factor(CNTF) on the regeneration of transected peripheral nerves Methods: Sciatic nerves in the middle of bilateral legs of 40 SD rats were resected for 5mm and the two cut ends were bridged by a silicone tube to form a regeneration chamber of nerve Gene recombination human CNTF(hCNTF) was injected into left chamber and 0 9% saline(NS) was in right Bridged nerves were exposed and observed at 14th,30th,60th,90th days after operation and the samples were observed by light and electron microscopes Image pattern analysis of axon,electro physiological examination and cholera toxin horse radish perxidase(CB HRP) mark test were done at 90th days after operation Results: The regenerated nerves of CNTF sides had more medullated fibers,larger mean axons diameters and thicker myelin sheath than those of control sides Incubation periods of complex muscle action potential(CMAP)of CNTF sides were shorter than that of control sides,nerve conductive velocity was quicker and wave amplitude higher Motor cells of cornu anterius medullae spinalis marked with CB HRP were more than that of control sides Conclusion: Exogenous hCNTE can promote regeneration of peripherial nerve.