大网膜内植入自体脾组织与原位脾组织的结构比较

目的 :为临床应用自体脾组织植入术提供实验研究资料。方法 :大鼠分为实验组和对照组 ,前者切取 1 /2脾脏去包膜后切成 1mm× 1mm× 1mm大小均匀组织块 ,植入大网膜囊袋内。饲养 6个月后取 2组脾组织制片 ,光镜和电镜定性观察组织结构变化 ,计算机图像分析系统比较血管、红髓、白髓及胶原纤维的面密度 ;免疫组化法结合计算机图像分析测定神经肽 (NPY)阳性神经纤维密度。结果 :神经和边缘窦内皮细胞结构恢复较好 ,血管 ,白髓的面密度值较对照组减少 ,红髓与对照组相当 ,胶原纤维面密度增加。结论 :大网膜内植入的自体脾组织通过再生能恢复脾脏的主要组织结构 ,但不能完全恢复正常。

Structure comparison of auto-implanted splenic tissue in greater omentum and normal spleen

Objective:To study tissue constructive difference between splenic auto-implanted tissue in greater omentum and normal spleen. Methods: Totally 40 healthy Wistar rats were randomly divided into experimental and normal control groups. In experimental group splenectomy and splenic auto-implantation in greater omentum were performed;in the control group only sham-operation was performed. After 6 monthes, tissue morphology changes of splenic autoimplants were investigated under light and transmission electron microscope; the NPY + nerve fibers were determined by immunohistochemical methods; area density of blood vessels, red pulp, white pulp and the NPY + nerve fibers were studied by computer image analysis system. Results: NPY + nerve fibers, marginal sinus endothelial cells structure were similar to normal spleen, but the area density of blood vessels, white pulp and NPY + nerve fibers were observably decreased (P<0.05), while those of collagenous fibre increased(P<0.05)and red pulp were not changed(P>0.05). Conclusion: Autoimplanted splenic tissue in greater omentum do not has the complete normal splenic tissue structure.