高温高湿环境下负压伤口疗法对创面细菌学定量的影响

目的探讨高温高湿环境下局部负压伤口治疗对创伤伤口的抗菌作用。方法以兔背部皮肤全层皮肤缺损的急性创面为模型,将24只创伤兔随机分为常温对照组、常温实验组、高温对照组、高温实验组,每组6只。实验组给予局部负压伤口治疗,对照组用纱布覆盖,不给予负压引流。观察伤口细菌数量的变化和伤口大体情况。结果高温对照组创面细菌量随着时间的延长呈直线上升趋势,与常温对照组、常温实验组比较差异有统计学意义（P〈0.05）,高温实验组细菌数量增长显著低于高温对照组（P〈0.05）。结论高温高湿环境有利于细菌生长繁殖,局部负压伤口疗法具有显著的隔离和抑制细菌生长的作用。

Influence of topical negative pressure wound therapy on bacteriological quantity of wounds in hot and humid environment

Objective To study the antibacterial effects of topical negative pressure wound therapy（TNP） on traumatic wound under high temperature and high humidity condition.Methods After the acute whole-skin-loss wounds model of rabbit backside were established,a total of 24 rabbits were divided into 4 groups equally（6 rabbits each） and randomly as normal temperature control（NEC）,ordinary temperature experiment（NEE）,high-temperature control（HHC）,and high-temperature experiment（HHE）.The rabbits of HHE and NEE were treated with TNP,and the rabbits of HHC and NEC were treated with normal dressing.Wound bacteriological ration and appearance were observed.Results Compared with the normal environment（NEC,NEE）,the bacteriological ration of HHC increased significantly after trauma and the difference had statistical significance（P〈0.05）.Compared with HHC,the increase of bacteriological ration in HHE was significantly lower after trauma（P〈0.05）.Conclusion Ahigh temperature and high humidity circumstance is one of important influence factors that cause the bacterial rise on wound.The wound bacteriological ration under high temperature and high humidity condition can be efficiently counteracted by topical negative pressure wound therapy.