抗精神病药奎硫平的神经保护作用

目的：探讨抗精神病药奎硫平的神经保护作用，阐明其神经保护作用的机制。方法：采用体外原代培养的大鼠海马神经元，复制谷氨酸(Glu)损伤模型以模拟抑郁症时的病理改变。实验分为正常对照组、Glu损伤组和奎硫平给药组。体外MTT法检测各组神经元存活率，酶学检测试剂盒测定不同浓度（50、100、200和500μmol·L-1）奎...

Neuroprotective effect of antipsychotic drug quetiapine

Objective To investigate the neuroprotective effect of antipsychotic drug quetiapine and to demonstrate the mechanism of neuprotective effect.Methods Primary cultured hippocampal neurons were injured with glutamate(Glu) to set up Glu-injured model and to simulate the pathological changes in depression.Three groups were included in the experiment:control group,Glu model group and quetiapine administration group.The survival rates in various groups were detected by MTT method;the release level of LDH,the activity of GSH,the activity of SOD,and the level of MDA were detected before and after treated with different doses of quetiapine(50,100,200 and 500 μmol·L-1) by enzyme detection kits.Results The survival rate of neuron in Glu model group was decreased compared with control group,almost 50% of normal data(P<0.05);but compared with Glu group,the rate was increased when the concentration of quetiapine was 200 μmol·L-1(P<0.05).The LDH release level in Glu model group was increased dramatically compared with control group,up to 7.4 times of control group(P<0.001);compared with Glu group,the LDH release level was decreased in quetiapine group(P<0.01).The GSH activity in Glu model group was decreased to 9.2 % of control group(P<0.05),which was increased in quetiapine group compared with Glu model group(P<0.05).The SOD activity in Glu model group was decresded significantly compared with control group(P<0.01),which was 3.8 times of that in Glu model group when the concentration of quetiapine was 10 μmol·L-1(P<0.001).The MDA level in Glu model group was increased,nevertheless,which was reduced in quetiapine administration group compared with Glu model group(P<0.01).Conclusion Quetiapine possesses the neuroprotection and the mechanism may involve that it can elevate the GSH activity and SOD activity as well as decrease the level of MDA.