电离辐射对人成纤维细胞中透明质酸酶表达的影响

目的:研究电离辐射对人成纤维细胞中透明质酸酶(HAase)表达的影响,并探讨其在放射性臂丛神经损伤致病机制中的作用.方法:0.5、1.0、2.0、4.0和6.0 Gy X射线分别照射人成纤维细胞24和48 h,同时设立假照组(0GyX射线),透射电镜观察其超微结构的改变,并采用ELISA法和Real-time PCR法...

Effect of ionizing radiation on expression of hyaluronidase in human fibroblasts

Objective To study the effect of ionizing radiation on the expression of hyaluronidase(HAase) in human fibroblasts and to discuss the role of HAase in the pathogenic mechanism of radiation-induced brachial plexus injury.Methods The fibroblasts were irradiated for 24 and 48 h respectively by X-ray with doses of 0.5,1.0,2.0,4.0 and 6.0 Gy,and sham irradiation group(0 Gy X-ray) was set up.The ultrastructural changes of fibroblasts were observed by transmission electron microscope,the expression changes of protein and mRNA of HAase were detected by ELISA and real-time PCR method.Results After irradiated for 48 h,the nuclear chromatin began to get together in 2.0 Gy group;the nucleus and chromosome fractured and the chromosome appeared empty bubble change in 4.0 Gy group;the chromatin was no longer apparent and the apoptosis change presented early in 6.0 Gy group.After irradiated for 24 and 48 h,with the increasing of the radiation dose,the expression of HAase protein declined,there was significant difference compared with sham irradiation group(P<0.05).The expressions of HAase mRNA in 0.5 and 4.0 Gy groups were significantly higher than that in sham irradiation group(P<0.05),but the expressions of HAase mRNA in 1.0,2.0 and 6.0 Gy groups were significantly lower(P<0.05) at 24 h.The expression of HAase mRNA in 2.0 Gy,4.0 Gy and 6.0 Gy groups were significantly higher than that in sham irradiation group at 24 h(P<0.05),the expressions of HAase mRNA in 0.5 Gy and 1.0 Gy groups were significantly lower at 48 h(P<0.05).Conclusion The increase of the radiation dose could reduce the expression of HAase in human fibroblasts,and influence the occurrence of radiation-induced brachial plexus injury.