3种真菌多糖对嗜酸乳杆菌发酵特性的影响

目的:研究姬松茸多糖、金针菇多糖和香菇多糖对嗜酸乳杆菌发酵特性的影响,对其能否作为新型益生元进行评价,筛选出3种真菌多糖作为益生元的最优添加浓度.方法:以嗜酸乳杆菌发酵乳作为研究对象,分别加入不同浓度的碳源.其中,实验组加入姬松茸多糖(浓度分别为0.2％、0.4％、0.6％、0.8％及1.0％),金针菇多糖(浓度分别为...

Effects of three kinds of fungi polysaccharides on fermentation characteristics of lactobacillus acidophilus

Objective To study the effects of ABM polysaccharide,flammulina velutipes polysaccharide and lentinan on the fermentation properties of lactobacillus acidophilus,and explore whether they can be considered as kinds of new prebiotics,and screen the best concentrations of the three fungi polysaccharides when they are used as prebiotics.Methods Fermented milk with lactobacillus acidophilus was studied by adding different concerntrations of carbon sources.Experimental groups were added with ABM polysaccharide(0.2%,0.4%,0.6%,0.8%,and 1.0%),flammulina velutipes polysaccharide(0.2%,0.4%,0.6%,0.8%,and 1.0%)and lentinan(0.4%,0.6%,0.8%,1.0%,and 1.2%),positive control group was added with the traditional prebiotics inulin(1%,2%,3%,4%,and 5%);the microbiological indexes,viscosities and pH values of all fermented milk samples were detected,and compared with negative control group.Results In the experiments of detecting microbial indicators,the viable count indexes were the largest when the ABM polyasccaride and flammulina velutipes polysaccharide concentrations were 0.6%,and the lentinan concentration was 0.8%,the results were 10.34,9.75 and 9.95 logcfu·mL-1;the viable counts of experimental groups were all larger than those in negative control group.In the experiments of detecting viscosity,the viscosity indexes were the largest when the ABM polyasccaride and lentinan concentrations were 0.8%,and flammulina velutipes polysaccharide concentration was 0.6%,the results were 3 800,3 300 and 2 800 mPa.s;the viscosities of experimental groups were all larger than those in negative control group.In the experiments of detecting pH,the pH values were the lowest when the ABM polyasccaride and flammulina velutipes polysaccharide concentrations were 0.6%,and the lentinan concentration was 0.8%,the results were 4.74,5.12 and 5.08;the pH values of experimental groups were all lower than those in negative control group.Conclusion ABM polysaccharide,flammulina velutipes polysaccharide and lentinan have influences in the fermentation properties of lactobacillus acidophilus.All of them can reach the standard of being prebiotics.ABM polyasccaride is the best prebiotics in this study.