survivin、环氧化酶-2及bcl-2在非霍奇金淋巴瘤中的表达及其相关性

 目的　研究survivin、环氧化酶-2（COX-2）及bcl-2在非霍奇金淋巴瘤（NHL）中的表达及其临床意义并初步探讨三者的相关性。方法　用免疫组织化学MaxVision法检测44例NHL和同期20例反应性淋巴样增生（RH）组织中survivin、COX-2及bcl-2蛋白的表达。结果　survivin、COX-2、bcl-2在NHL中的阳性表达率分别为70.45 %（31／44）、68.18 %（30／44）、63.64 %（28／44）；RH中分别为40.00 %（8／20）、40.00 %（8／20）和20.00 %（4／20），差异有统计学意义（均P＜0.05）。NHL组织中，COX-2与survivin（r＝0.306，P＝0.043）、survivin与bcl-2（r＝0.339，P＝0.040）的表达均呈正相关。结论　COX-2、survivin、bcl-2在NHL组织中呈高表达；survivin、bcl-2作为分子标志可能对淋巴瘤的诊断、分型具有一定的价值；COX-2和survivin、survivin和bcl-2在NHL的发生和发展中可能具有协同作用。

Expression and correlation of survivin, COX-2 and bcl-2 in non-Hodgkin lymphoma

Objective To investigate the expression of survivin,COX-2 and bcl-2 in non-Hodgkin lymphoma (NHL) and the significance and correlation between them.Methods Immunohistochemistry MaxVision systems for survivin,COX-2 and bcl-2 were conducted on 44 NHL and 20 reactive lymphoid hyperplasia (RH).Results The positive expression rates of survivin,COX-2,bcl-2 in NHL were 70.45 ％(31/44),68.18 ％ (30/44),63.64 ％ (28/44),respectively,and these in RH were 40.00 ％ (8/20),40.00 ％(8/20) and 20.00 ％ (4/20),respectively.There was positive correlation between the expression of COX-2 and survivin (r =0.306,P =0.043),survivin and bcl-2 protein (r =0.339,P =0.040) in NHL.Conclusion COX-2,survivin,bcl-2 are highly expressed in NHL.To detect the expression of them has clinical value to diagnosis NHL and to estimate the malignant degree of lymphoma.There are a positive correlations between the expression of COX-2 and survivin protein,and between the expression of survivin and bcl-2 protein,which indicates that they may play a synergistic role in the occurrence and development of NHL.