urantide对大鼠血管平滑肌细胞增殖的影响

目的:探讨在尾加压素Ⅱ(UⅡ)受体拮抗剂urantide干预下,UⅡ在大鼠血管平滑肌细胞(VSMC)中的表达,阐明其可能的作用机制.方法:采用贴块法对大鼠胸主动脉血管平滑肌细胞进行体外培养,实验分为正常对照组(C组)、UⅡ组(M组)、阳性药对照组(Flu组)及urantide干预组(浓度分别为1×10-10、1×10-...

Effect of urantide on proliferation of vascular smooth muscle cells in rats

Objective To investigate the expressions of both urotensin Ⅱ(UⅡ) and its receptor GPR14 in rat vascular smooth muscle cells(VSMC) under the intervention of urantide(UⅡ receptor antagonist),and clarify its possible mechanism.Methods The VSMC from rat thoracic aorta were cultivated in vitro with tissue explant method,and divided into normal control group(C group),UⅡ group(M group),positive control group(Flu group) and five intervention groups(10-10,10-9,10-8,10-7 and 10-6 mol·L-1 urantide).The proliferation index(PI) and S-phase cell fraction(SPF) of in vitro cultured VSMC were detected with MTT method and flow cytometry.Results Compared with C group,the VSMC proliferation was increased at each time point(P<0.01),and PI and SPF were also increased significantly(P<0.01);compared with M group,the VSMC proliferation in 10-10-10-6 mol·L-1 urantide groups were significantly decreased(P<0.05 or P<0.01),and PI and SPF were significantly reduced(P<0.05 or P<0.01),among which 10-6 mol·L-1 urautide group had the most significant effect.Conclusion Urantide can block the mitogenic effect of UⅡ on atherosclerosis(AS) of the major disease-promoting cells VSMC,providing a new perspective and experimental basis for clinical application of urantide to treat AS.