小白菊内酯对涎腺腺样囊性癌细胞系SACC-83的增殖抑制及其凋亡诱导作用

目的:探讨小白菊内酯(Par)对涎腺腺样囊性癌细胞系SACC-83的增殖抑制作用,并阐明其相关作用机制.方法:不同浓度(5.0、7.5、15.0、30.0、40.0和80.0 mg·L-1)Par作用于体外培养的SACC-83细胞系,同时设空白对照组；MTT比色法观察Par对SACC-83细胞系的生长抑制情况；流式细胞...

Proliferation inhibition and apoptosis induction effects of parthenolide on SACC-83 cells in vitro

Objective To investigate the inhibitory effect of proliferation of parthenolide(Par) on salivary gland adenoid cystic carcinoma cell line SACC-83 cells in vitro and clarify its related mechanism.Methods The SACC-83 cells were treated with different concentrations(5.0,7.5,15.0,30.0,40.0 and 80.0 mg·L-1) of Par in vitro,the proliferation inhibition of SACC-83 cells was detected by MTT colorimetry,the cell cycle distribution and apoptosis status were analyzed by flow cytometry,the expression of Livin protein was determined by Western blotting.Results The growth of SACC-83 cells in Par groups was inhibited effectively in concentration-and time-dependent manner.The apoptotic rates in Par groups were 14.7%±2.1%,30.5%±3.4% and 38.7%±2.7%,respectively,after treated with 7.5,15.0 and 30.0 mg·L-1 Par for 24 h,there were significant differences compared with control group(3.2%±0.9%,all P<0.01).The expressions of Livin proteins in Par groups were significantly down-regulated,there were significant differences compared with control group(P<0.05);and there were significant differences between different doses of Par groups(P<0.01).Conclusion Par could obviously inhibit the proliferation of SACC-83 cells.The expression of Livin protein may be related to this effect.