血管生成素2经PI3K/Akt途径对HCT-8细胞增殖的促进作用

目的:研究外源性血管生成素2(Ang-2)蛋白对结肠癌细胞株(HCT-8)中Tie-2、1-磷脂酰肌醇3-激酶(PI3K)、Akt基因及蛋白表达的影响,探讨其与PI3K/Akt通路的关系.方法:用不同浓度的Ang-2蛋白(0、0.2、0.4、0.8、1.2、2.0、3.0 mg·L-1)作用于HCT-8细胞,用MTT法...

Promoting effects of Ang-2 through PI3K/Akt pathways on proliferation of HCT-8 cells

Objective To observe the effects of angiopoietin-2(Ang-2) on the expressions of Tie-2,PI3K,Akt gene and protein in the colon cancer cell line(HCT-8) and analyze the relationship between Ang-2 and PI3K/Akt.Methods Different doses of Ang-2(0,0.2,0.4,0.8,1.2,2.0,and 3.0 mg·L-1) were added into the DMEM medium of HCT-8 cell line and the effective stimulus concentration of Ang-2 on HCT-8 was selected by MTT.The cells were divided into four groups: normal control group(C group),serum-free culture medium group(SD group),Ang-2 group(SA2 group) and LY294002 group(SA2L group),the gene and protein expressions of Tie-2,PI3K and Akt were detected by RT-PCR and Western blotting.Results Different concentrations of exogenous Ang-2 showed various proliferation effects on the HCT-8 and the most effective dose of Ang-2 was 2 mg·L-1.Compared with SD group,the expressions of Tie-2,PI3K and Akt gene and protein in SA2 group were increased significantly(Tie-2: all P<0.01;PI3K: P<0.01 or P> 0.05;Akt: P<0.01);compared with SA2 group,the expressions of three genes and proteins in SA2L group were decreased significantly(Tie-2: P<0.05 or P<0.01;PI3K: P<0.05 or P<0.01;Akt: P<0.01 and P<0.05).Conclusion Ang-2 can promote the proliferation of the colon cancer cells through the Ang-2/Tie-2/PI3K/Akt pathway which can be inhibited by LY294002.