牵张激活通道对膨胀心室大鼠原癌基因c-fos表达的影响

目的:研究牵张心室时牵张激活通道（SACs）对心肌细胞表达原癌基因c-fos的影响。方法:离体灌流大鼠心脏并膨胀左心室30 m in,停止灌流剪下左心室,抽提组织总RNA行半定量RT-PCR,探讨牵张左室对心室细胞表达c-fos的影响及SACs在其中的可能作用。结果:RT-PCR结果示,在同样牵张力度及持续时间膨胀心室的条件下,牵张加链霉素组c-fos表达水平明显低于无链霉素组。结论:SACs通道阻断剂-链霉素对膨胀左室致c-fos表达有明显抑制作用,间接说明心室膨胀经SACs激活胞内信号诱导c-fos表达;同时链霉素并不能完全抑制c-fos表达,提示膨胀心室致c-fos表达进而引起心室肥厚尚有其他传导途径,仍需进一步研究。

Effect of stretch-activated channels on ventricular dilation-induced c-fos expression in rats

AIM: To investigate the effects of stretch-activated channels(SACs) on ventricular c-fos expression.METHODS: Isolated normal adult rat hearts were perfused and streched by Langendorff method.The left ventricles were cut off after 30 min reperfusion.The total RNA was extracted for RT-PCR to determine c-fos expression.RESULTS: The level of c-fos expression in streptomycin group was significantly lower than that of the group without streptomycin.CONCLUSION: Streptomycin,a blocker of SACs,can partially inhibit left ventricular dilation-induced c-fos expression,impling that SACs may be one of the pathways to induce c-fos expression and ventricular hypertrophy.