Involvement of microRNA-21 in mediating chemoresistance to docetaxel in androgen-independent prostate cancer PC3 cells |
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Authors: | Guo-hai SHI Ding-wei YE Xu-dong YAO Shi-ling ZHANG Bo DAI Hai-liang ZHANG Yi-jun SHEN Yao ZHU Yi-ping ZHU Wen-jun XIAO Chun-guang MA |
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Institution: | [1]Department of Urology, Cancer Hospital, Fudan University, Shanghai 200032, China; [2]Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China |
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Abstract: | Aim:To investigate whether microRNA-21 was involved in mediating the chemoresistance of prostate cancer cells to docetaxel.Methods:A microarray technique was used to determine the miRNA profile in docetaxel-resistant PC3 cells. Real-time PCR was used to confirm the array results. miR-21 mimics and inhibitors were synthesized and introduced to cells using Lipofectamine 2000. Cell proliferation was examined with the CCK-8 assay. Luciferase reporter containing PDCD 3′UTR was constructed and the activity was detected by a dual luciferase assay. PDCD4 protein expression was evaluated using Western blot.Results:A docetaxel-resistant prostate cancer PC3 cell line (PC3R) was established . Using microarrays, miR-21 was found to be up-regulated in PC3R cells. Ectopic expression of miR-21 increased the resistance to docetaxel in PC3 wild type cells. In contrast, silencing of miR-21 in PC3R cells sensitized the cells to docetaxel. The IC50 values for miR-21-silencing cells and control cells were 28.31 and 35.89 nmol/L, respectively. PDCD4, a direct target gene of miR-21, could mediate chemoresistance to docetaxel in PC3 cells.Conclusion:Our findings suggest that miR-21 contributed to the resistance of PC3 cells to docetaxel, and that targeting miR-21 may offer a promising therapeutic approach in sensitizing prostate cancer to docetaxel treatment. |
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Keywords: | miR-21 chemoresistance docetaxel prostate cancer PDCD4 protein PC3 cells |
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