Indirect fluorescent antibody test for the diagnosis and therapeutic evaluation of trichinosis

ＩｎｄｉｒｅｃｔｆｌｕｏｒｅｓｃｅｎｔａｎｔｉｂｏｄｙｔｅｓｔｆｏｒｔｈｅｄｉａｇｎｏｓｉｓａｎｄｔｈｅｒａｐｅｕｔｉｃｅｖａｌｕａｔｉｏｎｏｆｔｒｉｃｈｉｎｏｓｉｓＣｕｉＪｉｎｇ崔晶，ＷａｎｇＺｈｏｎｇｑｕａｎ王中全，ＷｕＦｅｎｇ武峰，ＪｉｎＸｕｅ...

Indirect fluorescent antibody test for the diagnosis and therapeutic evaluation of trichinosis

Objective To evaluate the values of indirect fluorescent antibody test for the diagnosis and therapeutic evaluation of trichinosis.Methods Indirect fluorescent antibody test using frozen section of the purified Trichinella larvae as antigen was employed on the detection of anti-Trichinella antibodies in sera from the mice infected with T. spiralis and the patients with trichinosis. Sera from patients with trichinosis were collected at different time intervals after the onset of disease and after treatment with albendazole (20 mg/kg per day). Sera from the patients with other parasitic diseases and from healthy individuals were used as control.Results The anti-Trichinella antibodies were detected in the mice as early as 2 weeks after infection. The specific antibodies were observed in 86.38% of the of the patients with trichinosis. All sera from patients with filariasis, schistosomiasis and echinococcosis and from healthy controls were negative. The antibody positive rate of patients with trichinosis was only 70.59% one week after the onset of disease, and increased to 91.30%, 95.83% and 100% in 2, 3 and 4 weeks after the onset respectively. The frozen section antigens could be stored at -20℃ for at least 5 years without the loss of activity. After therapy, the detection time of antibodies was 1 to 2 weeks ahead of time. The antibody positive rate increased from 87.5% before therapy to 100% one week after therapy. Serum antibody titers one week after therapy were also obviously higher than those before therapy. The distinct elevation of serum antibody levels in a short term after therapy could be considered as an objective criterion of effective therapy. The antibody negative conversion rate of the patients was 24% one month after therapy, and increased to 75% four months after therapy.Conclusions Indirect fluorescent antibody test using frozen section of the purified Trichinella larvae as antigens is a higher sensitive, specific and stable technique and can be used to diagnose and evaluate the therapeutic effect of trichinosis.