Identification of two SNPs in myostatin (MSTN) gene of Takifugu rubripes and their association with growth traits |
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| Affiliation: | 1. Key Laboratory of Mariculture & Stock Enhancement in North China''s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China;2. The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences and Program of Cell and Molecular Biosciences, Auburn University, Auburn, AL 36849, USA;1. Department of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University, 50, Daehak-ro, Yeosu, Jeonnam 59626, Republic of Korea;2. Department of Horticulture, College of Life Science and Natural Resources, Sunchon National University, 255, Jungang-ro, Suncheon-Si, Jeollanam-do, 67922, Republic of Korea;1. Centre for Marine Sciences, CCMAR-CIMAR Laboratório Associado, Universidade do Algarve, Campus do Gambelas, 8005-139, Faro, Portugal;2. The Roslin Institute and R(D)SVS, University of Edinburgh, The Roslin Building, Easter Bush, Midlothian, EH25 9RG, United Kingdom;3. MPI Molecular Genetics, Ihnestrasse 63-73, D-14195 Berlin-Dahlem, Germany;4. Max-Planck-Institut für Pflanzenzüchtungsforschung Carl-von-Linné-Weg 10, D-50829 Köln, Germany;5. Laboratory of Applied Fish Genetics and Fish Breeding, Department of Aquaculture and Fisheries Management, Technological Educational Institute of Messolonghi, Nea Ktiria, 30200 Messolonghi, Greece;6. Laboratory of Biodiversity and Evolutionary Genomics, University of Leuven, Ch. Deberiotstraat 32, B-3000 Leuven, Belgium |
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| Abstract: | The myostatin (MSTN) is a member of transforming growth factor-β superfamily which inhibits muscle growth. In this study, the genomic DNA sequence of MSTN gene was cloned from Takifugu rubripes (T. rubripes). Two polymorphisms of the MSTN gene were detected by polymerase chain reaction-single strand conformation polymorphism (PCR–SSCP) and DNA sequencing methods in 296 T. rubripes. One A748G locates in exon 2 and the other, C1197T, in intron 2. Analysis showed that the A748G mutation caused an amino acid change from Thr to Ala (Ala166Glu). These two SNPs showed a low degree of linkage disequilibrium and four haplotypes were identified. The most frequent haplotype was AC, which occurred at a frequency of 44.3%. Association analyses between these two SNPs and growth traits showed that the individuals with genotype CT and TT of the mutation C1197T had significantly higher body mass, body length and body height than those with genotype CC (P < 0.05). These results show that MSTN gene can be utilized as a candidate gene for molecular marker-assisted breeding of T. rubripes. |
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| Keywords: | Myostatin (MSTN) PCR-SSCP SNP |
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