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Molecular cloning,characterization and expression of miR-15a-3p and miR-15b-3p in dairy cattle
Affiliation:1. College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling, Shaanxi 712100, China;2. PLA Air Force Xi''an Flight Academy, Huxian, Xi''an, Shaanxi 710306, China;1. Department of Life and Reproduction Sciences, University of Verona, Italy;2. ITAN: Italian Autism Network, Italy;1. Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Science, Faculty of Dental Science, Kyushu University, Japan;2. Maxillofacial Diagnostic and Surgical Sciences, Department of Oral and Maxillofacial Rehabilitation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan;1. USDA/ARS, 1636 E. Alisal St., Salinas, CA 93905, USA;2. Department of Biology, William Paterson University, Wayne, NJ 07470, USA;1. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China;2. Department of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan;3. Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan;4. Institute of Molecular Biology, National Chung Hsing University, Taichung 402, Taiwan;5. Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan;6. Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan;1. Institute of Molecular Biology, Acad. Roumen Tsanev, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Block 21, 1113 Sofia, Bulgaria;2. Department of Chemistry, Aristotle University of Thessaloniki, P. O. Box 135, 54124 Thessaloniki, Greece
Abstract:MicroRNA (miRNA) plays an important role in modulating immune responses. We identified miR-15a-3p and miR-15b-3p which were immune related in bovine. Fourteen potential target genes were predicted. Both miR-15a-3p and miR-15b-3p showed significant differential expression patterns in healthy and mastitis infected cattle (P < 0.01). Expression patterns of the two miRNAs provide important information on the relationships between mastitis and miRNA expression.
Keywords:Dairy cattle  Mastitis  Stem-loop qPCR
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