CD4+CDHigh25调节性T细胞及负调控因子白细胞介素-10与异基因造血干细胞移植后移植物抗宿主病的关系研究

 目的　研究异基因造血干细胞移植（allo-HSCT）后患者外周血CD+4 CDHigh25 调节性T细胞（Treg细胞）及细胞因子白细胞介素-10（IL-10）与急性移植物抗宿主病（aGVHD）的关系。方法　采用流式细胞术检测13例al1o-HSCT后患者外周血CD+4 CDHigh25 Foxp3High Treg细胞、CD+4 CDHigh25 CDLow127 Treg细胞占CD+4 T细胞的百分含量，同时用酶联免疫吸附法（ELISA）检测对应时期血清中IL-10的质量浓度。结果　13例患者均获得造血功能重建；aGVHD组CD+4 CDHigh25 CDLow127 ／CD+4 与CD+4 CDHigh25 Foxp3High／CD+4 比例均明显低于无GVHD组，差异有统计学意义（P＜0.01）；Ⅲ～Ⅳ度aGVHD亚组CD+4 CDHigh25 CDLow127／CD+4 与CD+4 CDHigh25 Foxp3High／CD+4 比例低于Ⅰ～Ⅱ度aGVHD亚组，但差异无统计学意义（P＞0.05）；相同组别间CD+4 CDHigh25 CDLow127／CD+4 与CD+4 CDHigh25 Foxp3High／CD+4 差异无统计学意义。aGVHD组的IL-10质量浓度明显低于无GVHD组，差异有统计学意义（P＜0.01）。Treg细胞与IL-10变化呈相关性（r＝0.925，P＜0.05）。结论　Treg细胞的水平与allo-HSCT后aGVHD的发生有密切关系；通过监测Treg细胞水平对临床早期诊断aGVHD及判断aGVHD预后、指导免疫调节剂的应用具有重要意义；CDLow127 可以作为Treg细胞表面的特异标志，推进对Treg细胞的检测及分离纯化；IL-10是一种重要的负调控因子；Treg细胞与IL-10的表达在aGVHD患者存在相关性，可能为Treg细胞的免疫抑制机制提供一定基础。

Association of CD+4 CDHigh25 regulatory T cells and the negative control factor IL-10 with the development of acute graft-versus-host disease after allogeneic hematopoietic cell transplantation

Objective To investigate the relationship between the CD+4 CDHigh25 regulatory T cells and cytokine IL-10 and acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods Flow cytometric was used to detect the percentage of CD+4 CDHigh25Foxp3High Treg cell and CD+4 CDHigh25 CDLow127 Treg cell in CD+4 T cells and at the same time ELISA was used to test the serum IL-10 levels in corresponding period. Results 13 patients have received hematopoietic function reconstruction. aGVHD group of CD+4 CDHigh25 CDLow127/CD+4 and CD+4 CDHigh25 Foxp3High/CD+4 ratio were significantly lower than non-aGVHD group, the difference was statistically significant (P ＜0.01); Ⅲ-Ⅳ degree of aGVHD subgroup was lower than Ⅰ - Ⅱ degree of aGVHD subgroup, but no statistical significance(P ＞0.05); the same as between-group CD+4 CDHigh25 CDLow127/CD+4 and the CD+4 CDHigh25 Foxp3High/CD+4 has no significant difference;aGVHD group of IL-10 concentration was significantly lower than non-GVHD group, the difference was statistically significant (P ＜0.01). Treg cell and IL-10 changes in correlation, r = 0.557, P ＜0.05. Conclusion The level of Treg cell was closely related to the occurrence of aGVHD after allo-HSCT. So it is very important to monitor the Treg cell level for clinical early diagnosis of aGVHD and predict prognosis of aGVHD and guide the application of immunosuppressant. CD127 can serve as a Treg cell surface-specific marker, to promote the detection of Treg cell and purification. IL-10 was an important negative regulator. Treg cell and IL-10expression in patients with aGVHD was correlation, which may provide some basis for Treg cell immunosuppressive mechanism.