低剂量γ射线辐照对血清抗氧化系统的兴奋效应

目的：探讨低剂量γ射线辐照人全血对血清抗氧化酶活性的影响。方法：20份人全血，以剂量为1Gy的γ射线进行辐照，吸收剂量率为17Gy/min，分别于照射前及照射后1、2h取样检测血清中总抗氧化能力（T—AOC）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）活力及还原性谷胱甘肽（GSH）、丙二醛（MDA）含量。结果：离体全血在剂量为1Gy γ射线照射后1h，可见血清中GSH含量升高，MDA含量降低及T—AOC、SOD、GR、GSH—Px、CAT活性升高，且均与照射前比较有显著差异（P〈0．01）。经γ射线照射2h后，除SOD活性与照射后1h差异无显著性外，MDA含量进一步降低，GSH含量及T—AOC、GR、GSH—Px及CAT活性进一步升高，与照射后1h比较有显著差异（P〈0．01）。结论：低剂量γ射线辐照能明显提高血清抗氧化能力，即可诱导抗氧化系统的兴奋效应。

Hormesis effect of serum antioxidative system after low dose human whole blood gamma ray irradiation

Objective：To investigate the activities of serum anti - oxidative enzymes by human whole blood gamma ray irradiation . Methods： Twenty units of human whold blood were irradiated by γ- ray at a dose of 1Gy. The absorption dose rate was 17 Gy/min. The serum enzymatic activities of total antioxidative capacity（T-AOC）, superoxide dismutase （ SOD ）, glutathione peroxidase （ GSH - Px ）, catalase （ CAT）, glutathionereductase （ GR ）, contents of glutathione （ GSH ） and malondiadehyde （ MDA ） were deteted before and 1,2 h after irradiation respectively. Results： One hour after irradiation, the content of serum GSI-I was higher than that of pre - radiation （P 〈0.01 ）. The content of serum MDA was lower than that of pre -radiation （p 〈 0.01 ）. But the enzymatic activities of T - AOC, SOD, GSH - Px, GR and CAT were higher than that of pre - radiation （p 〈 0.01 ）. In two hours after radiation group, the content of serum GSH was higher than that of one hour after radiation group （P 〈 0. 01 ）, but content of serum MDA was lower（P 〈0.01 ）. All antioxidative enzyme activities were higher than that of one hour after radiation group except SOD （p 〈 0.01 ）. Conclusion： Gamma ray irradiation at low dose can obviously enhance the serum antioxidative abilities, that is to say, it results in the hormesis of antioxidation system.