HPLC分离测定氨苄西林及其降解产物

 目的：建立新的HPLC分离测定氨苄西林及其降解产物的方法。方法：色语条件为：Nova-Pak C₁₈色谱柱；0.02 mol·L^-1磷酸二氢钠溶液(pH4.5)-乙腈(95：5)为流动相；检测波长为230 nm。结果：在0.24～1.20 mg·ml^-1的浓度范围内线性关系良好，r=0.9991，平均回收率为100.6%(n=5)，RSD=0.47%，重复进样RSD=0.06%(n=5)，氨苄西林及其降解产物得到基线分离。结论：本法简便、快速、准确，适用于氨苄西林的含量浏定及其降解产物的检查。

Determination of ampicillin and its degradation products by means of HPLC

OBJECTIVE: To develop a new HPLC method for the isolation and determination of ampicillin and its dergadation products. METHODS: Chromatographed conditions included column:Nova Pak C₁₈;mobile phase:0.02 mol·L^-1monobasic sodium phosphate (NaH₂PO₄) solution (pH4.5)acetonitrile (95∶5);detection wave lengths at 230 nm. RESULTS: There was a good linear relationship over the range of 0.24～1.20 mg·ml^-1,r=0.9991.The average recovery was 100.6% with RSD0.47%(n=5).The RSD for repetition was 0.06%(n=5)，ampicillin and its degradation products were isolated on base line. CONCLUSION: The method was simple,rapid,accurate and can be used to assay ampicillin and its degradation prouducts.