| 黄芩苷对小鼠肝细胞色素P450的选择性诱导 |
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| 引用本文： | 侯艳宁,程桂芳,朱秀媛.黄芩苷对小鼠肝细胞色素P450的选择性诱导[J].药学学报,2000,35(12):890-892. |
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| 作者姓名： | 侯艳宁 程桂芳 朱秀媛 |
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| 作者单位： | 1. 白求恩国际和平医院,石家庄,050082
2. 中国医学科学院,中国协和医科大学药物研究所,北京,100050 |
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| 摘 要： | 目的 观察黄芩苷对小鼠肝细胞色素P450及其亚家族的影响。方法 用紫外分光光度法分别测定小鼠肝微粒体细胞色素P450与b5含量及氨基比林N-脱甲基酶(ADM)、7-乙氧基香豆素O-脱乙基酶(ECD)、苯并芘羟化酶(AHH)活性。用蛋白印迹杂交技术鉴定细胞色素P450同功酶。结果 黄芩苷可使小鼠肝微粒体细胞色素P450含量显著增加,并使ADM,ECD及AHH 3种酶活力显著增强。对6种P450同功酶的鉴定结果显示,黄芩苷可选择性诱导1A1,2B1及2C11 3种同功酶,对细胞色素b5含量及3A2,2D1和2E1 3种同功酶无诱导作用。结论 黄芩苷对小鼠肝细胞色素P450有选择性诱导作用。
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| 关 键 词： | 黄芩苷 细胞色素P450 药酶诱导 |
| 收稿时间： | 2000-03-12 |
| 修稿时间： | : |
EFFECTS OF BAICALIN ON LIVER MICROSOMAL CYTOCHROME P450 SYSTEM |
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| HOU Yan-ning,ZHU Xiu-yuan,CHENG Gui-fang.EFFECTS OF BAICALIN ON LIVER MICROSOMAL CYTOCHROME P450 SYSTEM[J].Acta Pharmaceutica Sinica,2000,35(12):890-892. |
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| Authors: | HOU Yan-ning ZHU Xiu-yuan CHENG Gui-fang |
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| Institution: | Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. |
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| Abstract: | AIM: To investigate the effect of baicalin on liver microsomal cytochrome P450 system and the mechanism of liver protective action of baicalin. METHODS: Liver microsomal cytochrome P450, b5, aminopyrin N-demethylase (ADM), 7-ethoxycoumarin O-deethylase (ECD) and benzopyrene hydroxylase (AHH) activity were quantitated by UV chromatography. Activities of six cytochrome P450 isoforms were assayed with Western Blotting. RESULTS: Baicalin increased liver microsomal cytochrome. P450 level and ADM, ECD and AHH activity significantly. The three P450 isoforms, 1A1, 2B1 and 2C11, were also induced selectively by baicalin, but the b5 level, 3A2, 2D1 and 2E1 were not induced. CONCLUSION: Baicalin increases liver microsomal cytochrome P450 level and induces selectively 1A1, 2B1 and 2C11 of P450 isoforms in mice. |
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| Keywords: | cytochrome P450 baicalin |
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