进出口水产品中霍乱弧菌的检测与分析

目的监测并分析进出口水产品中的霍乱弧菌。方法采用传统两步增菌,平板划线,分离可疑单菌落,VITEK 2 Compact全自动微生物鉴定仪、普通PCR、实时荧光定量PCR和血清凝集试验确认分离菌株。结果从水产品样品分离的菌株全自动微生物鉴定仪鉴定结果相似度达97%,普通PCR和实时荧光定量PCR检测为霍乱弧菌,ctx毒素基因检测为阴性,血清凝集试验为非O1非O139群霍乱弧菌。结论从水产品中检出霍乱弧菌非流行株,但仍需要加强主动监测,预防疫情的暴发。

Detection and analysis of Vibrio cholerae from import and export aquatic products

Objective To monitor and analysis Vibrio cholerae from import and export aquatic products.Methods The suspicious single colonies by traditional two step enrichment and flat crossed separation was adopted.After isolation,the suspicious single colonies were identified by VITEK 2 Compact automated microbe identification analyzer,common PCR,real-time PCR and serological tests.Results The probability of the isolated strain was 97% by VITEK 2 Compact automated microbe identification analyzer.It was detected as Vibrio cholera by common PCR and real-time PCR and the primer and fluorescent probe were from industrial standard.The virulence gene ctx in the isolated strain was not detected by common PCR.It was confirmed as non-O1 and non-O139 Vibrio cholera by serum agglutination test.Conclusion Although one non-epidemic strain of Vibrio chloerae was detected from the monitoring aquatic products,the monitoring work should be strengthened for epidemic prevention.