人鼻咽癌DNA与EBV-W片段探针的核酸分子杂交研究

20例人鼻咽部组织DNA,经酶切后,与EBV-W(Bam H Ⅰ)片段探针作核酸分子杂交。Southern印迹杂交发现鼻咽低分化癌组织DNA,经Bam H Ⅰ酶切后出现6.7、5.3、3.1、2.0Kb的4条杂交带,呈现了与W片段相同顺序的阳性区带,而鼻咽良性疾患和胎儿鼻咽组织DNA均为阴性,此结果提示在人鼻咽癌组织中,确实存在EB病毒基因组。

A STUDY ON NUCLEIC ACID HYBRIDIZATION OF HUMAN NASOPHARYNGEAL CARCINOMA WITH EBV--W FRAGMENT PROBE

Nucleic acid molecular hybridization ofthe DNA of human nasopharyngeal tissuesfrom 20 cases was studied.14 of themwere nasopharyngeal carcinoma(NPC), 2cases malignant lymphoma,2 cases naso-pharyngitis,1 case papilloma and 1 casenasopharynx in a fetus.These DNA weredigested with the restriction enzyme BamHI in standard conditions,then the frag-ments were separated by electrophoresis on0.8% agarose gels and transferred to nitrocel-lulose filters by Southern blot method,and were hybridized with nick translated~(32)P-labelled EBV-W(Bam HI) probe.The autoradiograme showed that the naso-pharyngeal carcinoma had similarpattern of restriction enzyme and thatthe presence of 6.7. 5.3, 3.1 and 2.0Kb of four hybridizing bands wasobserved in the DNA of NPC. Thesepositive hybridizing bands had a geneticsequence homologous to EBV-W (BamHI) fragment.Hybridizing bands werenot observed in the DNA of non-cancernasopharyngeal tissue.The experimentalresults demonstrated the presence of EB-viral genomes in nasopharyngeal carcinomatissue. The close relationship betweenEB-virus and the carcinogenesis ofnasopharyngeal carcinoma was furtherevidenced by molecular hybridizationtechniques.This study has provided someexperimental evidence for the elucidationof the mechanism of carcinogenesis andpathogeny of nasopharyngeal carcinoma.