| IRM-2小鼠cDNA文库的全长cDNA序列分析 |
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| 引用本文： | 王芹,刘晓秋,宋力,孙志娟,杜利清,徐畅,王彦,刘强.IRM-2小鼠cDNA文库的全长cDNA序列分析[J].苏州大学学报(自然科学版),2012(5):613-618. |
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| 作者姓名： | 王芹 刘晓秋 宋力 孙志娟 杜利清 徐畅 王彦 刘强 |
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| 作者单位： | 北京协和医学院,中国医学科学院放射医学研究所生物室,天津市分子核医学重点实验室,天津300192 |
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| 基金项目： | 国家自然科学基金资助项目（30870583）; 中国医学科学院放射医学研究所学科发展基金资助项目（SF0822） |
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| 摘 要： | 目的分析IRM-2小鼠全长cDNA文库的cDNA序列。方法根据IRM-2小鼠的21条EST,从IRM-2小鼠全长cDNA文库测定基于该21条EST序列PCR扩增的全长cDNA序列,通过与GenBank基因库同源序列信息比对,分析全长cDNA序列和性质。结果根据21对引物PCR产物的拼接,获得5条全长cDNA序列,与小鼠已知基因不同源,而且得到了可能编码蛋白质的氨基酸序列和蛋白质的性质。结论 5条全长cDNA序列提示IRM-2小鼠体内可能存在辐射抗性相关基因,为进一步分离和鉴定辐射抗性相关基因奠定了基础。
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| 关 键 词： | IRM-2小鼠 cDNA文库 cDNA序列 氨基酸序列 |
Full-length cDNA sequence analysis on IRM-2 mouse cDNA library |
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| WANG Qin,LIU Xiao-qiu,SONG Li,SUN Zhi-juan,DU Li-qing,XU Chang,WANG Yan,LIU Qiang.Full-length cDNA sequence analysis on IRM-2 mouse cDNA library[J].Suzhou University Journal of Medical Science,2012(5):613-618. |
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| Authors: | WANG Qin LIU Xiao-qiu SONG Li SUN Zhi-juan DU Li-qing XU Chang WANG Yan LIU Qiang |
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| Institution: | , LI Jin,FAN Fei-yue(Dept of Biology,Institute of Radiation Medicine,Chinese Academy of Medical Sciences,Peking Union Medical College,Tianjin Key Laboratory of Molecular Nuclear Medicine,Tianjin 300192,China) |
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| Abstract: | Objective To analyse cDNA sequences from full-length cDNA library of IRM-2 mice.Methods Full-length cDNA products were amplified by PCR from cDNA library of IRM-2 mice according to twenty-one pieces of EST.Full-length cDNA sequences and properties were analyzed by comparing with GenBank database.Results Five pieces of full-length cDNA were obtained according to PCR products amplified by twenty-one pairs of primers,which were not the same as that of known mice genes.Amino acid sequences and properties of proteins encoded by five pieces of full-length cDNA were acquired.Conclusion Five pieces of full-length cDNA imply that radioresistance correlative genes may be existed in IRM-2 mice,laying a foundation for isolating and identifying radioresistance correlative genes in further study. |
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| Keywords: | IRM-2 mice cDNA library cDNA sequence amino acid sequence |
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