外周血MSCs的分离培养及对G-CSF的动员反应

目的建市多种种属外用血间充质干细胞（MSCs）的分离培养方法，探讨其对粒细胞集落刺激因子（G—CSF）的动员反应。方法用percoll（1．131g/mL）分离多种种属外周血单个核细胞，进行贴壁培养MSCs。以G-CSF作为动员剂，培养骨髓和外周血来源的MSCs，计数动员前后的成纤维细胞集落形成单位（CFU-F）。结果对多种种属进行外周血MSCs分离培养，成功率不同。在本实验条件下．可以稳定的直接分离培养出大鼠外周血的MSCs。G—CSF动员后，骨髓来源MSCs的CFU-F数昔显著高于对照组（P〈0．01）；外周血来源MSCs的CFU-F数量也显著高于对照组（P〈0．01），接近4倍。结论不同种属外周血MSCs分离培养难易不同，其直接原因是生理条什下外周血中存在的MSCs量少导致的。G—CSF可以有效地动员大鼠骨髓和外周血MSCs。

The feasibility analysis of MSCs mobilization by G-CSF and its prosthetic effection in traumatic brain injury

Objective To establish the isolating cultural method of MSCs from some genus peripheral blood(PB),and explore the mobilization response of MSCs by G-CSF.Methods MSCs from some genus PB were isolated by percoll(1.131 g/mL) and carried out adherent culture.MSCs-derived bone marrow and PB were cultured and its CFU-F were counted after mobilization by G-CSF.Results PB MSCs were isolated and cultureed successfully or unsuccessfully from different genus.Under our experiment condition,rat's PB MSCs could been isolated and cultured steadily.A significantly higher number of CFU-F of BM-derived MSCs and PB-derived MSCs than that of control group after mobilization by G-CSF(P<0.01),almost 4-fold increase.Conclusion PB MSCs were isolated and cultured successfully or unsuccessfully from different genus,it concerned with a small number of circulating MSCs in BP.We confirmed that G-CSF mobilized BM-derived MSCs,especially PB-derived MSCs by CFU-F assay.