血管抑素抑制人脑胶质瘤生长及病理学研究

目的：观察人血管抑素（Angiostatin, AS）对小鼠脑胶质瘤皮下移植瘤生长的抑制作用。方法：用MTT法观察AS对血管内皮细胞系ECV304和人脑胶质瘤细胞系TJ905增殖的影响；建立荷G422脑胶质瘤小鼠皮下移植模型，皮下注射AS，计算瘤体比和抑瘤率。结果： (1)AS对ECV304的抑制作用随着剂量的增加而增强，AS对TJ905无影响；(2)动物实验显示，AS剂量为10 mg·kg-1·d-1和50 mg·kg-1·d-1时抑瘤率分别为21.8%和84.3%；(3)免疫组化Ⅷ因子染色表明实验组与对照组之间在血管发育及数量方面均有差别。结论： AS在体外对胶质瘤细胞无抑制作用，在体内能通过抑制血管内皮细胞增殖而抑制胶质瘤的生长。

Inhibitory Effects of Angiostatin on the Growth of Human Glioma and It's Pathological Study

Objective:To investigate the inhibitory effects of human angiostatin ( AS) on the subcutaneously transplanted mouse glioma. Methods; Effects of AS on the proliferation of angioendothelial cell line ECV-304 and human glioma cell line TJ-905 were observed with MTT method. Mouse model of subcutaneously transplanted of glioma line G422 was established. AS was injected subcutaneously, tumor ratio and tumor in hibitory rate were estimated. Results; ( 1) Inhibitory effects of AS on ECV-304 enhanced with the increase of dose, while AS exerted no effect on TJ-905 ; (2) It was shown with animal experiments that turner inhibitory rates were 21. 8% and 84. 3% with AS doses of 10 mg and 50 mg-kg-1. d-1 , respectively; (3) Immunochemical factor VM staining indicated there the differences between the control and the control and the test groups in both the angiodevelopment and quantity. Conclusion; There was no inhibitory effect for AS to angiolorna cell in vitro, however in vivo, it inhibited the glioma growth via its inhibitory effect on the proliferation of an-gioend othelial cell line. It was then suggested that AS would be of wide application in tumor treatment.