| RP-HPLC测定茵栀黄软胶囊中绿原酸的含量 |
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| 引用本文： | 吴静,刘贤耀.RP-HPLC测定茵栀黄软胶囊中绿原酸的含量[J].现代中药研究与实践,2007,21(6):46-47. |
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| 作者姓名： | 吴静 刘贤耀 |
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| 作者单位： | 安徽省芜湖市医疗器械检测中心,安徽,芜湖,241000;安徽省芜湖市医疗器械检测中心,安徽,芜湖,241000 |
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| 摘 要： | 目的建立茵栀黄软胶囊中绿原酸含量的RP-HPLC测定方法。方法采用十八烷基硅烷键合硅胶柱,流动相:乙腈-0.4%磷酸溶液(13∶87),流速为1.0 mL.min-1,检测波长为327 nm。结果绿原酸线性范围:20~700μg/mL(r=0.999 9),精密度:RSD为0.21%(n=5),平均回收率为99.6%。结论试验表明,该方法灵敏度高,简便易行,结果准确,重现性好。
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| 关 键 词： | 茵栀黄软胶囊 绿原酸 RP-HPLC |
| 文章编号： | 1673-6427（2007）06-0046-02 |
| 收稿时间： | 2007-07-19 |
| 修稿时间： | 2007年7月19日 |
Determination of Chlorogenic Acid in Yinzhihuang Soft Capsules by HPLC |
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| WU Jing,LIU Xian-yao.Determination of Chlorogenic Acid in Yinzhihuang Soft Capsules by HPLC[J].Research and Practice on Chinese Medicines,2007,21(6):46-47. |
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| Authors: | WU Jing LIU Xian-yao |
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| Abstract: | Objective To develop a method for determination of chlorogenic acid in Yinzhihuang soft capsules.Methods RP-HPLC was used with C18 column,acetonitrile-0.4%phosphoric acid(13∶87)as mobil phase and detection wavelength was at 327nm,the flow rate was 1.0 mL· min-1,the flow rate was 1.0 mL·min-1.Results The calibration curve was linear in the range of 0.03～0.07 mg·mL-1(r=0.999 9).The average recovery was 99.6%,RSD was 1.4%(n=5).Conclusion The method appears to be simple,accurate and reproducible. |
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| Keywords: | Yinzhihuang soft capsules Chlorogenic acid RP-HPLC |
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