多不饱和脂肪酸对非酒精性脂肪性肝炎大鼠肝组织脂肪细胞因子的影响

目的 观察多不饱和脂肪酸对非酒精性脂肪性肝炎(NASH)大鼠肝组织中瘦素、抵抗素和脂联素的影响.方法 将44只大鼠随机分为4组,对照组(10只)给予普通饲料喂养,模型组(10只)、低剂量组(12只)和高剂量组(12只)给予高脂饲料喂养制备NASH模型.低剂量组和高剂量组在喂养8周后加用ω-3多不饱和脂肪酸干预(0.5 ...

The relation between polyunsaturated fatty acid and adipocytokines on NASH rats

Objective To observe the efficacy of polyunsaturated fatty acid（PUFA） on adipocytokines in rats with NASH. Methods Forty-four rats were randomly divided into 4 groups （control/ NASH/ lower therapy/ higher therapy group）. Rats in control group were fed with normal diet,and the other three with high-fat diet. co-3 unsaturated fatty acid was added to therapy group from 8th week to 12th week（0.5 g/d and 1.0 g/d）. The body/liver weight, HDL, LDL, TC and TG in liver of rats in each group were measured. Adipocytokines were detected by ELISA and real time fiourescence quantitive PCR. Results NASH model was established successfully. The weight, HDL, LDL, TC and TG were decreased in lower/ higher therapy group, as compared with those in rats with NASH. Changes of HDL, LDL and TC showed significant difference in higher therapy group（P〈0.05, P〈0.01, P〈0.01 ）. Lower/higher therapy group showed lower leptin, resistin and higher adiponectin levels than those in NASH group. Changes depended on dosage of PUFA. Adipocytokines in liver differed between higher group and lower one（P〈0. 05）, among which resistin and adiponectin mRNA expression was of statistical significance （P〈 0. 01 ）. Conclusion PUFA could improve lipid metabolism and regulate the expression of adipocytokines on NASH rats.