肠三叶因子对新生鼠坏死性小肠结肠炎Bim和Bcl-xl基因表达的影响及意义

目的 分析肠三叶因子(ITF)对坏死性小肠结肠炎(NEC)新生鼠肠黏膜组织Bim基因与Bcl-xl基因表达水平的影响,探讨ITF对NEC的保护作用机制.方法 采用简单随机法将30只新生鼠分为对照组、NEC组及ITF组,每组10只,对照组不作处理,NEC组建立NEC模型后予以腹腔注射生理盐水0.2ml;ITF组为NEC模型后予以腹腔注射ITF 0.2 mg.对切片行HE染色并作组织病理学检查及免疫组织化学法检测Bim基因与Bcl-xl基因表达,并进行图像分析.结果 病理切片显示NEC组肠壁损伤轻重不一,可见全肠黏膜绒毛坏死,病理组织学改变中位积分为3分;ITF组肠上皮细胞少量脱落,顶端绒毛坏死,病理组织学改变中位积分为1分;图像分析结果显示NEC组Bim基因表达(7.87±0.14)高于对照组(2.15±0.28)及ITF组(3.27±0.34),三组比较差异均有统计学意义(P<0.05);ITF组Bcl-xl基因表达(11.23±0.22)高于对照组(1.89±0.28)及NEC组(2.51±0.13),三组比较差异均有统计学意义(P<0.05).结论 通过腹腔注射ITF可减轻NEC大鼠的肠道损伤,而ITF可能通过改变Bim基因与Bcl-xl基因表达水平保护NEC大鼠肠道损伤.

Influence and significance of intestinal trefoil factor on Bim and Bcl-xl gene expression in neonatal rats with necrotizing enterocolitis

Objective To analyze the influence of intestinal trefoil factor(ITF) on Bim and Bcl-xl gene expression in neonatal rats with necrotizing enterocolitis(NEC),and to discuss the protective machanism of ITF on NEC.Methods Thirty neonatal rats were divided randomly into control group,NEC group and ITF group.NEC group were given intraperitoneal injection of saline 0.2 ml after NEC model of neonatal rats were established.ITF group were given intraperitoneal injection ITF 0.2mg after NEC model of neonatal rats were established.On the 4th day,all the subjects were put to death.We made HE stainting of the slice and made a histopathological examination and immunohistochemical method to detect Bim and Bc1-xl genes expression,and make image analysis.Results The pathological lesions indicated that intestinal tissue necrosis was severe in NEC group,which median was 3 point,but obviously lessen in ITF group,which median was 1 point,with ITF interfering.Image analysis showed the NEC group Bim gene expression (7.87 ± 0.14) higher than those in the control group (2.15±0.28) and ITF group (3.27±0.34),there were significant differences between 3 groups(P<0.05).Bcl-xl gene expression(11.23±0.22)in ITF group was higher than that in control group(1.89±0.28) and NEC group(2.51±0.13),there were significant differences between 3 groups(P<0.05).Conclusions Intestinal injury was ameliorated after ITF was injected intraperitoneally,ITF may protect the intestinal injury of neonatal rats with NEC by changing the Bim gene and Bc1-xl gene expresstion ratio.