| 半边莲生物碱对内皮素诱导损伤的人血管内皮细胞纤溶系统的影响 |
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| 引用本文： | 范秀珍,王婧婧,任冬梅,肖颖,陈融,李莉,胡维诚. 半边莲生物碱对内皮素诱导损伤的人血管内皮细胞纤溶系统的影响[J]. 山东大学学报(医学版), 2005, 43(10): 898-901 |
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| 作者姓名： | 范秀珍 王婧婧 任冬梅 肖颖 陈融 李莉 胡维诚 |
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| 作者单位： | 山东大学,护理学院,山东,济南,250012;山东大学,医学院病理生理学教研室,山东,济南,250012;山东大学,药学院天然药化教研室,山东,济南,250012;山东大学,医学院分子医学实验室,山东,济南,250012 |
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| 基金项目： | 教育部高等学校博士学科点专项科研基金资助项目（20030422067）,山东省自然科学基金资助项目（Y2004C32） |
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| 摘 要： | 目的：研究半边莲生物碱对内皮素诱导损伤的人血管内皮细胞的保护作用。方法：常规提取半边莲生物碱，培养人血管内皮细胞．ELISA法测定细胞培养上清中组织纤溶酶原激活物（t-PA）和纤溶酶原激活物抑制物-1（PAI-1）的含量。结果：内皮素组PAI-1含量显著高于正常对照组（P〈0．01）．t-PA含量在数值上低于正常对照组，但无统计学意义。半边莲生物碱各组PAI-1含量均低于内皮素组（P〈0．01）．与内皮素B受体拮抗剂BQ7S8作用相似。半边莲生物碱低剂量组t-PA含量高于内皮素组（P〈0．01）．高、中剂量组t-PA含量虽然有增高趋势．但无统计学意义。结论：内皮素能够使人血管内皮细胞释放PAI-1增加．半边莲生物碱通过抑制该效应而起到保护血管内皮细胞的作用。
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| 关 键 词： | 半边莲生物碱 内皮素 内皮细胞 组织型纤溶酶原激活物 纤溶酶原激活物抑制物-1 |
| 文章编号： | 1671-7554（2005）10-0898-04 |
| 收稿时间： | 2004-04-13 |
| 修稿时间： | 2005-05-08 |
Influence of Lobelia Chinensis Lour alkaloids on fibrinolysis system of human vascular endothelial cells treated with endothelin |
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| FAN Xiu-zhen,WANG Jing-jing,REN Dong-mei,XIAO Ying,CHEN Rong,LI Li,HU Wei-cheng. Influence of Lobelia Chinensis Lour alkaloids on fibrinolysis system of human vascular endothelial cells treated with endothelin[J]. Journal of Shandong University:Health Sciences, 2005, 43(10): 898-901 |
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| Authors: | FAN Xiu-zhen WANG Jing-jing REN Dong-mei XIAO Ying CHEN Rong LI Li HU Wei-cheng |
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| Affiliation: | 1. School of Nursing; 2.Department of Pathophysiology, School of Medicine;3.Department of Natural Pharmaceutical Chemistry, School of Pharmaceutical Sciences ;4. Molecular Medical Laboratory, School of Medicine, Shandong University, Jinan 250012, Shandong, China |
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| Abstract: | Objective: To observe the injury effect of endothelin on human vascular endothelial cells(HVECs) and study the inhibitory role of Lobelia Chinensis Lour alkaloids (LCLAs) on this effect. Methods: Alkaloids were extracted from Lobelia Chinensis Lour. The concentrations of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) in supernatants were assayed using the enzyme linked immunosorbent assay technique. Results: The concentrations of PAI-1 were higher in endothelin groups than in control group (P<0.01). LCLAs lessened the release of PAI-1 significantly from HVECs injured by endothelin (P<0.01). Their effects were similar to those of endothelin B receptor antagonist,BQ788. Conclusion: LCLAs can protect HVECs against the injury of endothelin. |
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| Keywords: | Lobelia Chinensis Lour alkaloids Endothelin Endothelial cells Tissue-type plasminogen activator Plasminogen activator inhibitor-1 |
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