Apoptosis induced by α-anordrin in human promyelocytic leukemia HL-60 cells

Induction of apoptosis in human promyelocytic leukemia HL-60 cells by α-anordrin was estimated with the use of morphological and biochemical methods. α-Anordrin 2.5-50.0 μmol·L^-1 inhibited the growth of HL-60 cells in a dose-dependent manner and arrested them at G₁ phase of cell cycle. The cells exhibited marked morphological changes such as cell shrinkage, nuclear fragmentation, chromatin condensation. Agarose gel electrophoresis of DNA extracted from α-anordrin-treated cells revealed a “ladder” pattern. Nearly 63% of cells underwent apoptosis at α-anordrin concentration of 50 μmol·L^-1 as determined by flow cytometry. The data demonstrate that α-anordrin induces HL-60 cell apoptosis, which might be related to its antitumor action.

Apoptosis induced by α-anordrin in human promyelocytic leukemia HL-60 cells

Induction of apoptosis in human promyelocytic leukemia HL-60 cells by α-anordrin was estimated with the use of morphological and biochemical methods. α-Anordrin 2.5-50.0 μmol·L^-1 inhibited the growth of HL-60 cells in a dose-dependent manner and arrested them at G₁ phase of cell cycle. The cells exhibited marked morphological changes such as cell shrinkage, nuclear fragmentation, chromatin condensation. Agarose gel electrophoresis of DNA extracted from α-anordrin-treated cells revealed a “ladder” pattern. Nearly 63% of cells underwent apoptosis at α-anordrin concentration of 50 μmol·L^-1 as determined by flow cytometry. The data demonstrate that α-anordrin induces HL-60 cell apoptosis, which might be related to its antitumor action.