| 3例疑难ABO血型鉴定及分析 |
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| 引用本文： | 左宏莉,徐群丽,郭伟鹏,廖声凯,吐尔洪·克维尔.3例疑难ABO血型鉴定及分析[J].新疆医科大学学报,2007,30(9):990-992. |
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| 作者姓名： | 左宏莉 徐群丽 郭伟鹏 廖声凯 吐尔洪·克维尔 |
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| 作者单位： | 1. 新疆维吾尔自治区血液中心输血研究所,新疆,乌鲁木齐,830000
2. 新疆维吾尔自治区老年病医院,新疆,乌鲁木齐,830000 |
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| 摘 要： | 目的:鉴定并分析3例疑难ABO血型红细胞抗原及血清抗体的特异性。方法:运用PCR-SSP方法对3例患者ABO血型抗原进行基因分型;运用血型血清学技术对造成ABO血型正反定型不符的原因进行分析。结果:3例患者ABO血型基因分型分别为A/O、O/O、O/O。结合血型血清学技术为患者提供了安全的血液制品。结论:ABO血型基因分型技术弥补了血型血清学方法的一些不足,运用血型基因分型技术可以解决血清学方法难以鉴定的血型抗原。
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| 关 键 词： | ABO血型 聚合酶链反应-序列特异性引物(PCR-SSP) 基因分型 血型血清学 |
| 文章编号： | 1009-5551（2007）09-0990-03 |
| 修稿时间： | 2007-05-17 |
Examination and analysis of ABO blood group in three doubt patients |
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| ZUO Hong-li, XU Qun-li, GUO Wei-peng, et al.Examination and analysis of ABO blood group in three doubt patients[J].Journal of Xinjiang Medical University,2007,30(9):990-992. |
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| Authors: | ZUO Hong-li XU Qun-li GUO Wei-peng |
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| Institution: | Depatment of Blood Institute, Blood Centre of Xinjiang Uighur Autonomous, Urumqi 830000, China |
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| Abstract: | Objective: Examination and analysis of red cell antigens and specificity of serum-antibody of ABO blood group in three doubt patients. Methods: To use the methods of polymerase chain reacation sequence specific primer for genetyping of red cell antigens of ABO blood group in three doubt patients, to analyse the reason of disparity for ABO blood group detection in blood group serology. Results: The results of genetyping are A/O, O/O, O/O in ABO blood group of three doubt patients. Combine with blood group serology to provide safe blood products for patients. Conclusion: To make up the deficient of blood group serology method, the technology of gnetyping in ABO blood group was used. It is difficult to detect in blood group serology method, but the genetype of blood group can solve it. |
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| Keywords: | ABO blood group polymerase chain reacation sequence specific primer (PCR-SSP) genetyping blood group serology |
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