聚合酶链反应技术在淋菌性尿道炎诊断中的价值

目的探讨聚合酶链反应(PCR)技术对淋菌性尿道炎的诊断价值.方法320例淋菌性尿道炎患者分别采用PCR法及涂片(Smear)法染色进行检测,并对阳性检出率进行比较.结果320例中PCR法、Smear法阳性检出率分别为92%、 56%,PCR法明显高于Smear法(P＜0.01).结论PCR法检测淋菌优于涂片法,并具有快速、特异性高、敏感性强等特点,对临床诊断及治疗有重要的指导意义.

The value of polymerase chain reaction (PCR) for the diagnosis of gonorrhea urethritis

Objective: To estimate the value of the application of PCR in laboratory diagnosis for gonorrhea urethritis. Methods: Three hundred and twenty samples with gonorrhea urethritis were detected by PCR and smear simultaneously, and then compared with the results of the two detecting means. Results: All 320 male samples were detected by PCR and smear simultaneously. 294 of 320 samples were detected by PCR showed positive results, with positive rate 92%, and 84 of 320 samples were detected by smear showed positive results,with positive rate 56%.There was significant difference between detecting gonorrhea urethritis by PCR method and smear method. Conclusions: The PCR has an obvious advantage over smear to diagnose the gonorrhea urethritis in men. It also possesses the advantages as being fast, sensitive and specific. It has the guiding significance for diagnosis and selecting appropriate therapeutic protocol in patients.