人肺巨细胞癌PLA-801C和PLA-801D的体内自发转移性再研究

目的　了解人肺巨细胞癌亚系PLA -80 1C和PLA- 80 1D目前的体内自发转移特性,为进行下一步研究提供具体数据及所需模型。方法　采用肿瘤细胞动物皮下接种的方法,建立肿瘤自发性转移动物模型,实验结束时处死动物行病理学检查,统计成瘤率及转移率。结果　两株细胞的成瘤率均为10 0 %。腋窝皮下接种组PLA- 80 1C和PLA -80 1D的淋巴结转移率分别为0 ,33 .33% ,肺转移率分别为10 0 % ,6 6. 6 7%。左后腿皮下接种PLA 80 1D的裸鼠淋巴结及肺部的转移率分别为16. 6 7% ,4 1 .6 7%。已与前人所验证的结果明显不同。结论　本文分析了这两株细胞的成瘤性和转移性变化的原因,并认为这两株细胞在转移特性上仍存在明显差异,仍具有用于肿瘤转移领域研究的重要价值。

Restudy the in vivo spontaneous metastasis of human pulmonary giant cell carcinoma cell sub lines PLA-801C and PLA-801D

Objective To study the in vivo spontaneous metastasis of human pulmonary giant cell carcinoma cell sub lines PLA-801C and PLA-801D in order to afford animal models and specific data for further study. Methods Established animal spontaneous metastatic modals of subcutaneously transplanted PLA-801C and PLA-801D. The tumorigenicity and metastasis of transplanted tumor cells were judged histologically after sacrifice. Results The tumorigenicity of the two sub lines in nude mice were 100%. The metastasis rates to lymph nodes and lung were 0 and 100% for PLA-801C, 33.33% and 66.67% for PLA-801D transplanted to axillary space, and 16.67%, 41.67% for PLA-801D transplanted to hind legs. These results were different from which made by our predecessors. Conclusion The reason responsible for the change of the tumorigenicity and metastasis are discussed. The PLA-801C and PLA-801D are still be of great significance for the study of tumor metastasis.