| 钩端螺旋体DNA疫苗pcD-flaB的构建及其免疫机制的初步研究 |
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| 引用本文： | 寇志华,张平武,孙树汉. 钩端螺旋体DNA疫苗pcD-flaB的构建及其免疫机制的初步研究[J]. 第二军医大学学报, 2000, 21(6): 511-514 |
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| 作者姓名： | 寇志华 张平武 孙树汉 |
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| 作者单位： | 第二军医大学基础医学部医学遗传学教研室,上海,200433 |
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| 基金项目： | 国家863计划资助!项目 (10 1-0 6 -0 5 0 4) |
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| 摘 要： | 观察钩端螺旋本ＤＮＡ疫苗的保护效果,并探讨疫苗的免疫机制。方法：应用定向克隆的方法构建ＤＮＡ疫苗ｐｃＤ－ｆｌａＢ,肌肉途径免疫豚鼠,观察豚鼠攻击感染钩体后保护率,以ＥＬＩＳＡ法检测特异性抗体ＩｇＧ工观察疫苗对豚鼠腹腔巨噬细胞分泌的ＴＮＦ活性的影响。结果该疫苗对同型钩体的保护率为１００％,特异性抗体水平在疫苗注射第６周达到高峰,ＴＮＦ的活性明显增高。结论：ＤＮＡ疫苗ｐｃＤ－ｆｌａＢ对同型钩体感染有较
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| 关 键 词： | 钩端螺旋体 DNA 疫苗 巨噬细胞 pcD－flaB |
| 修稿时间： | 】 |
Construction of DNA vaccine pcD-flaB against pathogenic leptospira infection and its immune mechanism |
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| KOU Zhi-Hua,ZHANG Ping-Wu,SUN Shu-Han. Construction of DNA vaccine pcD-flaB against pathogenic leptospira infection and its immune mechanism[J]. Former Academic Journal of Second Military Medical University, 2000, 21(6): 511-514 |
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| Authors: | KOU Zhi-Hua ZHANG Ping-Wu SUN Shu-Han |
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| Abstract: | Objective:To investigate the immume mechanism and protective effect of DNA vaccine pcD flaB against pathogenic leptospira infection. Methods: DNA vaccine pcD flaB was constructed by inserting flaB into the eucaryotic expressing plasmid pcDNA3. After guinea pigs were infected with leptospira, the protective rate was observed and specific anti leptospira antibody IgG were tested by ELISA. TNF activity was tested by cell proliferation. Results:The protective rate against leptospira infection was 100%. The specific antibody IgG generated peaked at the 6th week. Activity of TNF released by macrophage of guinea pigs given DNA vaccine was higher than that not given vaccine. Conclusion:DNA vaccine pcD flaB can protect guinea pigs from leptospira challenge infection by inducing humoral immune response and increasing TNF activity. |
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| Keywords: | leptospira DNA vaccines macrophage T |
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