2007年山西省部分地区虫媒病毒调查

目的 调查山西省部分地区的虫媒病毒,在当地采集蚊虫标本进行病毒的分离与鉴定.方法 2007年7月和8月在当地采集蚊虫标本,通过组织细胞培养进行病毒分离,利用分子生物学和生物信息学技术对病毒分离物进行鉴定与分析.结果 分离到10株病毒分离物,鉴定结果显示分离株SX0765、SX0766、SX0767、SX0771、SX0789、SX0790、SX0793、SX0794、SX0795、SX0796均为版纳病毒;此外,分离株SX0771和SX0794还同时存在辽宁病毒基因.进一步分析显示版纳病毒新分离株与此前在北京、云南和辽宁分离到的病毒株之间存在明显差异,同源性在89.7%～94.1%之间.结论 在山西省分离到10株版纳病毒和两株辽宁病毒,版纳病毒与我国其他地区分离株有明显差异,在进化上相对独立.

Arbovirus investigation in some regions of Shanxi province in 2007

Objective To investigate arbeviruses in some regions of Shanxi province, isolation and identification for arbovirus activity from mosquitoes was conducted. Methods Mosquitoes were collected from these area in 2007 and then used for virus isolation by cell culture. The virus isolates were identified by molecular biology and the sequences were analyzed by bioinformaties. Results Ten Banns virus (strains SX0765, SX0766, SX0767 ,SX0771, SX0789, SX0790, SX0793, SX0794, SX0795, SX0796) were iselated, and two Liaoning virus were also isolated from isolates SX0771, SX0794. Phylogenetic tree of the Banns virus isolates showed that ten strains are located in a distinct branch from all of the other Chinese Banns virus isolates. The homology is between 89.7 % and 94.1%. Conclusion Ten Banna virus and two Lianning virus were isolated during this arbovirus investigation in Shanxi province. New Banns virus isolates showed a distinct phylogenetic relationship with the other Chinese Banns virus strains.